LSBio
TCP1 Antibody (C-Terminus, clone 91a)

TCP1 Antibody (C-Terminus, clone 91a)

Primary AntibodyLSBio Guarantee
Antibody:Rat Monoclonal
Applications:Flow Cytometry, Immunohistochemistry (general), Immunoprecipitation, Western Blot
Reactivity:Bovine, Dog, Fruit Fly, Guinea Pig, Hamster, Helminth / Nematode, Human, Mouse, Non-Human Primate, Pig, Plant, Rat
Format:Liquid
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$401.00each
Catalog Number: LS-C63149-50

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Overview

Description
TCP1 antibody LS-C63149 is an unconjugated rat monoclonal antibody to TCP1 (C-Terminus) from mouse. It is reactive with human, mouse, rat and other species. Validated for Flow, IP and WB.
Application
FC, IHC, IP, WB
Reactivity
Bov, Can, Dros, GP, Ham, Helm, Hu, Ms, NHP, Plnt, Por, Rt
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Monoclonal Antibodies
Alternative Names
CCTA | CCT-alpha | D6S230E | T-complex 1 | TCP-1-alpha | Tailless complex polypeptide 1 | CCT1 | TCP1
Recommended Dilution: FC
1:100
Recommended Dilution: WB
1:1000
UniProt Number
P17987
SwissProt
P17987

Target

Target
Mouse TCP1
Antigen Species
Mouse
Epitope
Recognizes an epitope in the C- terminus of mouse TCP-1a (465-AKLRA) and will detect an ~60 kD protein, corresponding to the apparent molecular mass of TCP1 on SDS-PAGE immunoblots, in samples from human, mouse, rat, bovine, canine, guinea pig, hamster, monkey, pig, and rabbit origins, as well as Saccharomyces cerevisiae (weakly, consistent with the epitope sequence being AKLRS), Drosophila, Schizosaccharomyces pombe and C. elegans. In C. elegans CTA-191 reacts with TCP-1a and another CCT subunit (2). In plants, CTA-191 recognizes TCP -1 of Pisum sativum, and the sequence of Arabidopsis thalania TCP-1a over the region of the epitope AKLRA. It has also been shown that CTA-191 reacts with a subunit of a specialized chaperonin which folds phytochrome (3). Cross reactivity with human Hsp60 has been observed with this antibody in immunoblot analysis.
Gene Name
TCP1

Antibody

Host Species
Rat
Clonality
Monoclonal
Clone
91a
Isotype
IgG2a

Immunogen

Immunogen
A purified recombinant mouse TCP-1 construct encoding the C-Terminus half of the 1.8 kb full-length Tcp1b gene expressed in E. coli.
Immunogen Type
Recombinant Immunogen

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
PBS, pH 7.2, 0.1 mM PMSF, 50% Glycerol
Preservative
No
Purification Method
Protein G Affinity

Storage & Handling

Storage Conditions
Store at -20°C.
Recommended Storage Buffer
PBS

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.