LSBio
TCP1 Antibody (C-Terminus, clone 23c)

TCP1 Antibody (C-Terminus, clone 23c)

Primary AntibodyLSBio Guarantee
Antibody:Rat Monoclonal
Applications:Immunocytochemistry, Immunohistochemistry (general), Immunoprecipitation, Western Blot
Reactivity:Bovine, Dog, Hamster, Mouse, Rabbit, Rat, Sheep
Format:Liquid
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$405.00each
Catalog Number: LS-C63148-50

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Overview

Description
TCP1 antibody LS-C63148 is an unconjugated rat monoclonal antibody to TCP1 (C-Terminus) from mouse. It is reactive with mouse, rat, bovine and other species. Validated for ICC, IHC, IP and WB.
Application
ICC, IHC, IP, WB
Reactivity
Bov, Can, Ham, Ms, Rb, Rt, Sh
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Monoclonal Antibodies
Alternative Names
CCTA | CCT-alpha | D6S230E | T-complex 1 | TCP-1-alpha | Tailless complex polypeptide 1 | CCT1 | TCP1
Recommended Dilution: WB
1:1000
UniProt Number
P17987
SwissProt
P17987

Target

Target
Mouse TCP1
Antigen Species
Mouse
Epitope
Detects a 60 kD protein, corresponding to the apparent molecular mass of TCP-1 alpha on SDS-PAGE immunoblots, in samples from mouse, rat, bovine, sheep, dog, rabbit and hamster origins. This antibody recognizes the C-terminus of mouse TCP-1 (LDDCOOH) but not TCP-1 of primates (LNDCOOH). The COOH group is probably recognized since addition of an alanine (LDDACOOH) prevents binding in ELISA assays to immobilized synthetic peptide sequences. This antibody recognizes other proteins, most notably the p102B' COP subunit of Golgi coatomer. CTA-123 binds protein A and is useful for native precipitations of coatomer and CTT. This antibody does not react with human Hsp60 protein. This antibody also detects a molecular mass band of approximately 92 kD on immunoblots.
Gene Name
TCP1

Antibody

Host Species
Rat
Clonality
Monoclonal
Clone
23c
Isotype
IgG2c

Immunogen

Immunogen
A purified recombinant mouse TCP-1a construct encoding the C-Terminus half of the 1.8 kb full-length Tcp1b gene expressed in E. coli.
Immunogen Type
Recombinant Immunogen

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
PBS, pH 7.2
Preservative
No
Purification Method
Protein G Affinity

Storage & Handling

Storage Conditions
Store at -20°C.
Recommended Storage Buffer
PBS

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.