Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~41.6 kDa TCF7 protein using Rabbit Anti-TCF7 Polyclonal Antibody. Lane 1: Molecular Weight Ladder (MW). Lane 2: Cervical Cancer cell line (HeLa) lysate. Load: 10 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-TCF7 Polyclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~41.6 kDa. Other Band(s): ~54 kDa isoform.

TCF7 Antibody (RPE)

Primary AntibodyLSBio Guarantee

Antibody:	Rabbit Polyclonal
Applications:	Immunohistochemistry (general), Western Blot
Reactivity:	Human
Format:	Liquid

$487.00each

Catalog Number: LS-C774712-100

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Overview

Description: TCF7 antibody LS-C774712 is an RPE-conjugated rabbit polyclonal antibody to human TCF7. Validated for IHC and WB.
Application: IHC, WB
Reactivity: Hu
Intended Use: Research Use Only
Guarantees: This antibody carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names: TCF-7 | TCF1 | TCF7 | Transcription factor 7 | T-cell factor 1 | TCF-1
Recommended Dilution: IHC: 1:50
Recommended Dilution: WB: 1:1000
UniProt Number: P36402
NCBI GenBank: NM_003202 | NP_003193.2
SwissProt: P36402

Target

Target: Human TCF7
Antigen Species: Human
Epitope: Detects 42 kDa, additional band at ~54 kDa is another isoform.
Gene Name: TCF7

Antibody

Host Species: Rabbit
Clonality: Polyclonal
Origin Species: Human

Immunogen

Immunogen: Synthetic peptide from the C-Terminus of Human TCF7 (284-384 aa), conjugated to Keyhole Limpet Haemocyanin (KLH).
Immunogen Type: Synthetic Peptide (Linear)