Immunofluorescence staining of TBXT in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with mouse anti-Human T monoclonal antibody (dilution ratio 1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-mouse IgG secondary antibody (green). Positive staining was localized to Nucleus.
TBXT / T / Brachyury Antibody (clone 07, Preservative Free)
Primary AntibodyLSBio Guarantee
| Antibody: | Mouse Monoclonal |
| Applications: | Immunocytochemistry, Immunofluorescence |
| Reactivity: | Human |
| Format: | Liquid |
$309.00each
Catalog Number: LS-C819504-100
Catalog Number: LS-C819504
For bulk orders or custom formulations:
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- Description
- Brachyury antibody LS-C819504 is an unconjugated mouse monoclonal antibody to human Brachyury (TBXT / T). Validated for ICC and IF.
- Application
- ICC, IF
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Alternative Names
- Brachyury | Brachyury protein | Protein T | TFT | T | T brachyury (mouse) homolog | T brachyury homolog | T, brachyury homolog (mouse) | TBXT
- Recommended Dilution: IF/ICC
- 1:20 - 1:100
- UniProt Number
- O15178
- NCBI GenBank
- NM_003181 | NP_003172.1
- SwissProt
- O15178
Target
- Target
- Human TBXT / T / Brachyury
- Antigen Species
- Human
- Epitope
- Human Brachyury
- Gene Name
- TBXT
Antibody
- Host Species
- Mouse
- Clonality
- Monoclonal
- Clone
- 07
- Isotype
- IgG2b
- Origin Species
- Human
Immunogen
- Immunogen
- A synthetic peptide corresponding to the C-Terminus of Human Brachyury/TBXT
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.2 µm filtered solution in PBS
- Volume
- 100 Microliter
- Preservative
- No
- Purification Method
- Protein A Affinity
Storage & Handling
- Storage Conditions
- Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.
- Recommended Storage Buffer
- PBS