SM22a was immunoprecipitated using: Lane A: 0.5 mg Jurkat Whole Cell Lysate. 1 uL anti-SM22a rabbit polyclonal antibody and 15 ul of 50% Protein G agarose. Primary antibody: Anti-SM22a rabbit polyclonal antibody, at 1:500 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 23 kDa. Observed band size: 23 kDa.
TAGLN / Transgelin / SM22 Antibody (Preservative Free)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Paraffin, Immunoprecipitation, Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$309.00each
Catalog Number: LS-C815439-100
Catalog Number: LS-C815439
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- Description
- SM22 antibody LS-C815439 is an unconjugated rabbit polyclonal antibody to human SM22 (TAGLN / Transgelin). Validated for ICC, IF, IHC, IP and WB.
- Application
- ICC, IF, IHC-P, IP, WB
- Reactivity
- Hu
- Predicted Reactivity
- Mouse, Rat
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- 22 kDa actin-binding protein | SMCC | TAGLN | TAGLN1 | WS3-10 | Transgelin | Transgelin variant 2 | SM22 | Protein WS3-10 | SM22-alpha
- Recommended Dilution: IF/ICC
- 1:1500 - 1:50000
- Recommended Dilution: IHC-P
- 1:2000 - 1:10000
- Recommended Dilution: WB
- 1:500 - 1:1000
- UniProt Number
- Q01995
- NCBI GenBank
- NM_003186 | NP_003177.2
- SwissProt
- Q01995
Target
- Target
- Human TAGLN / Transgelin / SM22
- Antigen Species
- Human
- Epitope
- Human Transgelin/TAGLN
- Gene Name
- TAGLN
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Human
Immunogen
- Immunogen
- A synthetic peptide corresponding to the C-Terminus of Human TAGLN
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.2 µm filtered solution in PBS
- Volume
- 100 Microliter
- Preservative
- No
- Purification Method
- Protein A Affinity
Storage & Handling
- Storage Conditions
- Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.
- Recommended Storage Buffer
- PBS