Western blot of rat cortex lysate showing specific immunolabeling of the ~78k synapsin I phosphorylated at Ser9 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphates: I-Ptase). The blot is identical to the control except that it was incubated in I-Ptase (1200 units for 30 min) before being exposed to the phospho-Ser9 synapsin I antibody. The immunolabeling is completely eliminated by the treatment with I-Ptase.
SYN1 / Synapsin 1 Antibody (phospho-Ser9)
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| Antibody: | Rabbit Polyclonal |
| Applications: | Western Blot |
| Reactivity: | Bovine, Human, Mouse, Rat, Xenopus |
| Format: | Liquid |
$479.00each
Catalog Number: LS-C223-100
Catalog Number: LS-C223
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- Description
- Synapsin 1 antibody LS-C223 is an unconjugated rabbit polyclonal antibody to Synapsin 1 (SYN1) (pSer9) from rat. It is reactive with human, mouse, rat and other species. Validated for WB.
- Application
- WB
- Reactivity
- Bov, Hu, Ms, Rt, Xen
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- Brain protein 4.1 | Synapsin | Synapsin I | Synapsin-1 | SYN1 | SYN1a | SYN1b | SYNI | p-Ser9 Synapsin I
- Recommended Dilution: WB
- 1:1000
- UniProt Number
- P17600
- NCBI GenBank
- NM_006950 | NP_008881.2
- SwissProt
- P17600
Target
- Target
- Rat SYN1 / Synapsin 1
- Antigen Species
- Rat
- Epitope
- Specific for ~78k synapsin I doublet protein phosphorylated at Ser9. The antibody also weakly labels the ~55k synapsin II protein which has a similar phosphorylation site to that of Ser9 on synapsin I. Immunolabeling is blocked by preadsorption of the antibody with the phosphopeptide used as antigen but not by the corresponding dephosphopeptide. Immunolabeling is also completely eliminated by treatment with lambda phosphatase.
- Gene Name
- SYN1
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
Immunogen
- Immunogen
- A phosphopeptide corresponding to amino acid residues surrounding the phosphoSer9 of synapsin I.
- Immunogen Type
- Peptide
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 10 mM HEPES, pH 7.5, 150 mM NaCl, 0.1 mg/mL BSA, 50% Glycerol
- Volume
- 100 Microliter
- Preservative
- No
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Short term: -20°C; Long term: -20°C.
- Recommended Storage Buffer
- 10 mM HEPES|150 mM NaCl