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Streptococcus pyogenes CRISPR-associated endonuclease Cas9/Csn1 Antibody (C-Terminus)

Western Blot of rabbit Anti-Cas9 Antibody. Lane 1: 293T non transfected cell lysate. Lane 2: 293T Cas9 over expressed cell lysate. Load: 15µg per lane. Primary Antibody cas9 used at 1µg/mL using MB-070 overnight at 4°C. Secondary Antibody: goat anti-rabbit HRP at 1:40,000 for 30 min at room temp. Expect: 158kDa.

Streptococcus pyogenes CRISPR-associated endonuclease Cas9/Csn1 Antibody (C-Terminus)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunofluorescence, Western Blot
Reactivity:Bacteria
Format:Liquid
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$579.00each
Catalog Number: LS-C745371-100

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Overview

Description
Csn1 antibody LS-C745371 is an unconjugated rabbit polyclonal antibody to streptococcus pyogenes Csn1 (Streptococcus pyogenes CRISPR-associated endonuclease Cas9) (C-Terminus). Validated for ELISA, IF and WB.
Application
ELISA, IF, WB
Reactivity
Bac
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Recommended Dilution: ELISA
1:25000 - 1:30000
Recommended Dilution: IF/ICC
1 µg/ml
Recommended Dilution: WB
1:1000

Target

Target
Streptococcus pyogenes CRISPR-associated endonuclease Cas9/Csn1
Antigen Species
Bacteria

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Isotype
IgG

Immunogen

Immunogen
Cas-9 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the C-terminal of Streptococcus pyogenes Cas-9 protein.
Immunogen Type
Synthetic Peptide (Linear)

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration
1 mg/mL
Preservative
Yes
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
Recommended Storage Buffer
0.02 M Potassium Phosphate|0.15 M NaCl

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