Rabbit Anti-Streptavidin Antibody - Dot Blot. Dot Blot showing the detection of Streptavidin. A three-fold serial dilution of DyLight 488 Conjugated Streptavidin starting at 200 ng was spotted onto 0.45 um nitrocellulose and blocked in 1% BSA-TTBS (MB-013, diluted to 1X) 30 min at 20°C. Anti-Streptavidin (RABBIT) Antibody (p/n LS-X35533) was incubated in Blocking Buffer for Fluorescent Western Blot (p/n MB-070) at 1:1000 for 1 Hour at 20°C. An HRP Gt-a-Rb secondary antibody was incubated at 1:40000 for 30 min at 20°C and imaged using the Bio-Rad VersaDoc 4000 MP.