LSBio
STAT5A Antibody (phospho-Tyr694, clone 5F6.F1)

Anti-Stat5 pY694 Monoclonal Antibody - Immunohistochemistry. Immunohistochemistry using Anti-Stat5 pY694 monoclonal antibody shows detection of phosphorylated Stat5pY694 in human breast tissue (40X). The antibody was used a dilution to 20 ug/mL. The image shows breast epithelium with moderate nuclear staining. Tissue was formalin fixed and paraffin embedded. Antigen retrieval: steam sections in 0.1 M sodium citrate buffer, pH 6, for 20 min. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain. Personal communication, Andrew Elston, Lifespan Biosciences, Seattle, WA.

STAT5A Antibody (phospho-Tyr694, clone 5F6.F1)

Primary AntibodyLSBio Guarantee
Antibody:Mouse Monoclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunofluorescence, Immunohistochemistry (general), Western Blot
Reactivity:Mouse
Format:Liquid
See all Mouse STAT5A antibodies →
$586.00each
Catalog Number: LS-C154006-100

For bulk orders or custom formulations:

Request a Quote

Overview

Description
STAT5A antibody LS-C154006 is an unconjugated mouse monoclonal antibody to STAT5A (pTyr694) from mouse. It is reactive with human, mouse and rat. Validated for ELISA, IF, IHC and WB.
Application
ELISA, IF, IHC, WB
Reactivity
Ms
Predicted Reactivity
Human, Rat
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Monoclonal Antibodies
Alternative Names
STAT5A | MGF | STAT5
Recommended Dilution: ELISA
1:20000
Recommended Dilution: IF/ICC
1:50 - 1:1000
Recommended Dilution: WB
1:500 - 1:2000
UniProt Number
P42229
SwissProt
P42229

Target

Target
Mouse STAT5A
Antigen Species
Mouse
Epitope
This antibody is specific for mouse Stat5a protein phosphorylated at Y694. A BLAST analysis was used to suggest cross-reactivity with Stat5a from human, mouse and rat based on 100% homology with the immunizing sequence. Cross-reactivity with Stat5a from other sources has not been determined.
Gene Name
STAT5A

Antibody

Host Species
Mouse
Clonality
Monoclonal
Clone
5F6.F1
Isotype
IgG1
Light Chain
Kappa Light Chain

Immunogen

Immunogen
Anti-STAT5 phospho Y694 monoclonal antibody was produced by repeated immunizations with a synthetic peptide corresponding to residues surrounding Y694 of mouse STAT5a protein.
Immunogen Type
Synthetic Peptide (Linear)

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration
1 mg/mL
Preservative
Yes
Purification Method
Protein A Affinity

Storage & Handling

Storage Conditions
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer
0.02 M Potassium Phosphate|0.15 M NaCl

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.