Anti-Stat1 pY701 Antibody - Western Blot. Western blot of Protein A purified anti-Stat1 pY701 antibody shows detection of phosphorylated Stat1 (indicated by arrowhead at ~91 kD) in K562 cells after 30 min treatment with 1Ku of hIFN-??(lane 2). No reactivity is seen for non-phosphorylated Stat1 in untreated cells (lane 1). The membrane was probed with the primary antibody at a 1:1000 dilution at 4C, overnight. Personal Communication from Ana Gamero, CCR-NCI, Bethesda, MD.

STAT1 Antibody (phospho-Tyr701)

Primary AntibodyLSBio Guarantee

Antibody:	Rabbit Polyclonal
Applications:	Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Western Blot
Reactivity:	Mouse
Format:	Liquid

$579.00each

Catalog Number: LS-C59556-100

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Overview

Description: STAT1 antibody LS-C59556 is an unconjugated rabbit polyclonal antibody to STAT1 (pTyr701) from mouse. It is reactive with mouse and rat. Validated for ELISA and WB.
Application: ELISA, IHC, WB
Reactivity: Ms
Predicted Reactivity: Human, Rat
Intended Use: Research Use Only
Guarantees: This antibody carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names: ISGF-3 | STAT91 | STAT1 | CANDF7
Recommended Dilution: ELISA: 1:25000
Recommended Dilution: WB: 1:500 - 1:2000
UniProt Number: P42224
NCBI GenBank: NM_007315 | NP_009330.1
SwissProt: P42224

Target

Target: Mouse STAT1
Antigen Species: Mouse
Epitope: This antibody is specific for mouse Stat1 protein phosphorylated at Y701. A BLAST analysis was used to suggest cross-reactivity with Stat1 from mouse and rat based on 100% homology with the immunizing sequence. Partial reactivity is expected against Stat1 from human sources as 90% homology is noted. Cross-reactivity with Stat1 from other sources has not been determined.
Gene Name: STAT1

Antibody

Host Species: Rabbit
Clonality: Polyclonal
Isotype: IgG

Immunogen

Immunogen: This Protein A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to residues surrounding Y701 in the mouse STAT1 protein.
Immunogen Type: Synthetic Peptide (Linear)

Format

Conjugate/Label: Unconjugated
Format: Liquid
Formulation: 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration: 2.3 mg/mL
Preservative: Yes
Purification Method: Protein A Affinity

Storage & Handling

Storage Conditions: Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer: 0.02 M Potassium Phosphate|0.15 M NaCl