Western blot analysis of extracts of various cell lines, using SSPN antibody at 1:3000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 90s.
SSPN / Sarcospan Antibody
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Western Blot |
| Reactivity: | Human, Mouse, Rat |
| Format: | Liquid |
$281.00each
Catalog Number: LS-C747357-20
Catalog Number: LS-C747357
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- Description
- Sarcospan antibody LS-C747357 is an unconjugated rabbit polyclonal antibody to Sarcospan (SSPN) from human. It is reactive with human, mouse and rat. Validated for WB. Cited in 1 publication.
- Application
- WB
- Reactivity
- Hu, Ms, Rt
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- DAGA5 | Kirsten-ras-associated protein | Kras oncogene-associated gene | Microspan | NSPN | Kras oncogene-associated | SSPN | Nanospan | SPN1 | SPN2 | KRAG | Sarcospan
- Recommended Dilution: WB
- 1:500 - 1:2000
- UniProt Number
- Q14714
- NCBI GenBank
- NM_005086 | NP_005077.2
- SwissProt
- Q14714
Target
- Target
- Human SSPN / Sarcospan
- Antigen Species
- Human
- Gene Name
- SSPN
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Human
Immunogen
- Immunogen
- Recombinant fusion protein containing a sequence corresponding to amino acids 1-60 of human SSPN (NP_005077.2). MGKNKQPRGQQRQGGPPAADAAGPDDMEPKKGTGAPKECGEEEPRTCCGCRFPLLLALLQ
- Immunogen Type
- Fusion Protein (e.g., GST, MBP tag)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol
- Volume
- 20 Microliter
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store at -20°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- PBS
Sarcospan increases laminin-binding capacity of a-dystroglycan to ameliorate DMD independent of Galgt2. Hafsa Mamsa, Rachelle L Stark, Kara M Shin, Aaron M Beedle, Rachelle H Crosbie. Human molecular genetics. 2022 March;31:718-732. PubMed: 34581784 PMC: PMC8895749