Immunoprecipitation(IP) of SSB by using monoclonal anti-SSB antibodies (Negative control: IP without adding anti-SSB antibody.). For each experiment, 500ul of DDK tagged SSB overexpression lysates (at 1:5 dilution with HEK293T lysate), 2 ug of anti-SSB antibody and 20ul (0.1 mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
SSB / La Antibody (clone OTI3H9)
Primary AntibodyLSBio Guarantee
| Antibody: | Mouse Monoclonal |
| Applications: | Flow Cytometry, Immunofluorescence, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Immunoprecipitation, Western Blot |
| Reactivity: | Dog, Human |
| Format: | Liquid |
$443.00each
Catalog Number: LS-C114516-100
Catalog Number: LS-C114516
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- La antibody LS-C114516 is an unconjugated mouse monoclonal antibody to La (SSB) from human. It is reactive with human and dog. Validated for Flow, IF, IHC, IP and WB.
- Application
- FC, IF, IHC, IHC-P, IP, WB
- Reactivity
- Can, Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Alternative Names
- Autoantigen La | La | La ribonucleoprotein | Lupus La antigen | SS-B | SS-B/La protein | SSB | La autoantigen | LARP3 | Lupus La protein
- Recommended Dilution: FC
- 1:100
- Recommended Dilution: IF/ICC
- 1:100
- Recommended Dilution: IHC-P
- 1:150
- Recommended Dilution: WB
- 1:1000 - 1:2000
- UniProt Number
- P05455
- NCBI GenBank
- NM_003142 | NP_003133.1
- SwissProt
- P05455
Target
- Target
- Human SSB / La
- Antigen Species
- Human
- Epitope
- Human SSB / La
- Gene Name
- SSB
Antibody
- Host Species
- Mouse
- Clonality
- Monoclonal
- Clone
- OTI3H9
- Isotype
- IgG2a
Immunogen
- Immunogen
- Protein expressed in 293T cell transfected with human SSB expression vector.
- Immunogen Type
- Cell-Based Immunization
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol, 1% BSA
- Concentration
- 1.2 mg/mL
- Volume
- 100 Microliter
- Preservative
- Yes
- Purification Method
- Protein A/G Affinity
Storage & Handling
- Storage Conditions
- Store at -20°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- PBS