LSBio
SQSTM1 Antibody (Biotin)

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-SQSTM1 Polyclonal Antibody. Tissue: Fibroblast cell line (NIH 3T3). Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-SQSTM1 Polyclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm, Late Endosome, Lysosome, Cytoplasmic Vesicle, Autophagosome, Nucleus, Endoplasmic Reticulum. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) SQSTM1 Antibody (D) Composite.

SQSTM1 Antibody (Biotin)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Immunofluorescence, Western Blot
Reactivity:Human, Mouse, Rat
Format:Liquid
See all Human SQSTM1 antibodies →
$487.00each
Catalog Number: LS-C774085-100

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Overview

Description
SQSTM1 antibody LS-C774085 is a biotin-conjugated rabbit polyclonal antibody to SQSTM1 from human. It is reactive with human, mouse and rat. Validated for IF and WB.
Application
IF, WB
Reactivity
Hu, Ms, Rt
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
A170 | ORCA | OSIL | Paget disease of bone 3 | p60 | p62 | PDB3 | Sequestosome-1 | Ubiquitin-binding protein p62 | Oxidative stress induced like | p62B | ZIP3 | Sequestosome 1 | SQSTM1 | EBI3-associated protein p60 | EBIAP
Recommended Dilution: IF/ICC
1:100
Recommended Dilution: WB
1:1000
UniProt Number
Q13501
SwissProt
Q13501

Target

Target
Human SQSTM1
Antigen Species
Human
Epitope
Detects ~60 kDa. Band at 40 kDa is isoform 2.
Gene Name
SQSTM1

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Origin Species
Human

Immunogen

Immunogen
Synthetic peptide from the N-Terminus to mid of Human SQSTM1 (aa. 195-205)
Immunogen Type
Synthetic Peptide (Linear)

Format

Conjugate/Label
Biotin
Format
Liquid
Formulation
PBS, 0.09% Sodium Azide, 50% Glycerol
Concentration
1 mg/mL
Preservative
Yes
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Store at -20°C.
Recommended Storage Buffer
PBS

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