Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-beta 4 Spectrin Monoclonal Antibody, Clone S393-2. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-beta 4 Spectrin Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) beta 4 Spectrin Antibody. (D) Composite.

SPTBN4 Antibody (clone S393-2, RPE)

Primary AntibodyLSBio Guarantee

Antibody:	Mouse Monoclonal
Applications:	Immunofluorescence, Immunohistochemistry (general), Western Blot
Reactivity:	Human, Mouse, Rat
Format:	Liquid

$487.00each

Catalog Number: LS-C773154-100

Unit Size

Quantity

For bulk orders or custom formulations:

Overview

Description: SPTBN4 antibody LS-C773154 is an RPE-conjugated mouse monoclonal antibody to SPTBN4 from human. It is reactive with human, mouse and rat. Validated for IF, IHC and WB.
Application: IF, IHC, WB
Reactivity: Hu, Ms, Rt
Intended Use: Research Use Only
Guarantees: This antibody carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Monoclonal Antibodies
Alternative Names: Beta-IV spectrin | KIAA1642 | SPNB4 | SPTBN3 | SPTBN4 | QV | Spectrin beta chain, brain 3
Recommended Dilution: WB: 1:1000
UniProt Number: Q9H254
NCBI GenBank: NM_025213 | NP_079489.2
SwissProt: Q9H254

Target

Target: Human SPTBN4
Antigen Species: Human
Epitope: Detects at about 200 kDa. Does not cross react with other Beta-spectrins
Gene Name: SPTBN4

Antibody

Host Species: Mouse
Clonality: Monoclonal
Clone: S393-2
Isotype: IgG1
Origin Species: Human

Immunogen

Immunogen: Fusion protein amino acids 1621-1832 (C-Terminus repeats 14 to 15) of human Beta4-spectrin
Immunogen Type: Fusion Protein (e.g., GST, MBP tag)