Detection of Human and Mouse SPTAN1/Alpha II-Spectrin by Western Blot. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug), 293T (T; 50 ug), and mouse NIH3T3 (M; 50 ug) cells. Antibody: Affinity purified rabbit anti-SPTAN1/Alpha II-Spectrin antibody used for WB at 0.2 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
SPTAN1 / Alpha Fodrin Antibody (aa1000-1050)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Immunohistochemistry (general), Western Blot |
| Reactivity: | Human, Mouse |
| Format: | Liquid |
$301.00each
Catalog Number: LS-C288062-10
Catalog Number: LS-C288062
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- Description
- Alpha Fodrin antibody LS-C288062 is an unconjugated rabbit polyclonal antibody to Alpha Fodrin (SPTAN1) (aa1000-1050) from human. It is reactive with human and mouse. Validated for IHC and WB.
- Application
- IHC, WB
- Reactivity
- Hu, Ms
- Predicted Reactivity
- Chicken, Rat
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- Alpha-II spectrin | Alpha Fodrin | Fodrin alpha chain | EIEE5 | NEAS | Alpha-fodrin | SPTAN1 | Spectrin alpha chain, brain | SPTA2
- Recommended Dilution: IHC
- 1:500 - 1:2000
- Recommended Dilution: WB
- 1:2000 - 1:10000
- UniProt Number
- Q13813
- NCBI GenBank
- NM_003127 | NP_003118.2
- SwissProt
- Q13813
Target
- Target
- Human SPTAN1 / Alpha Fodrin
- Antigen Species
- Human
- Epitope
- Region between residue 1000 and 1050 of human spectrin, alpha, non-erythrocytic 1 using the numbering given in entry NP_003118.1 (GeneID 6709).
- Gene Name
- SPTAN1
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
Immunogen
- Immunogen
- A portion of GeneID: 6709 corresponding to amino acids between 1000 and 1050 of UniprotID: Q13813
- Immunogen Type
- Recombinant Protein Fragment
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- Tris-citrate/phosphate buffer, pH 7-8, 0.09% sodium azide.
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store at 2-8°C for up to one year.
- Recommended Storage Buffer
- Tris-citrate