HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SPP1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SPP1.
SPP1 / Osteopontin Antibody (aa17-314, clone OTI6C12)
Primary AntibodyLSBio Guarantee
| Antibody: | Mouse Monoclonal |
| Applications: | Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$443.00each
Catalog Number: LS-C339892-100
Catalog Number: LS-C339892
For bulk orders or custom formulations:
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- Description
- Osteopontin antibody LS-C339892 is an unconjugated mouse monoclonal antibody to human Osteopontin (SPP1) (aa17-314). Validated for WB.
- Application
- WB
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Alternative Names
- BSPI | BNSP | Bone sialoprotein 1 | ETA-1 | Nephropontin | OPN | Secreted phosphoprotein 1 | SPP-1 | SPP1/CALPHA1 fusion | Urinary stone protein | Uropontin | Osteopontin | Bone sialoprotein I | SPP1
- Recommended Dilution: WB
- 1:2000
- UniProt Number
- P10451
- NCBI GenBank
- NM_000582 | NP_000573.1
- SwissProt
- P10451
Target
- Target
- Human SPP1 / Osteopontin
- Antigen Species
- Human
- Epitope
- Human SPP1 / Osteopontin
- Gene Name
- SPP1
Antibody
- Host Species
- Mouse
- Clonality
- Monoclonal
- Clone
- OTI6C12
- Isotype
- IgG1
Immunogen
- Immunogen
- Human recombinant protein fragment corresponding to amino acids 17-314 of human SPP1(NP_001035147) produced in E. coli.
- Immunogen Type
- Recombinant Protein Fragment
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol, 1% BSA
- Concentration
- 1 mg/mL
- Volume
- 100 Microliter
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store at -20°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- PBS