IHC staining of FFPE human spleen with SOX9 antibody (clone PU1/2146). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
SPI1 / PU.1 Antibody (clone PU1/2146, Azide-free, BSA-free)
Primary AntibodyLSBio Guarantee
| Antibody: | Mouse Monoclonal |
| Applications: | Immunohistochemistry, Paraffin, Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$586.00each
Catalog Number: LS-C782439-100
Catalog Number: LS-C782439
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- Description
- PU.1 antibody LS-C782439 is an unconjugated mouse monoclonal antibody to human PU.1 (SPI1). Validated for IHC and WB.
- Application
- IHC-P, WB
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Alternative Names
- 31 kDa-transforming protein | 31 kDa transforming protein | PU.1 | SFPI1 | SPI-1 | SPI-1 proto-oncogene | SPI-A | Transcription factor PU.1 | SPI1
- Recommended Dilution: IHC-P
- 1 - 2 µg/ml
- Recommended Dilution: WB
- 0.5 - 1 µg/ml
- UniProt Number
- P17947
- NCBI GenBank
- NM_003120 | NP_003111.2
- SwissProt
- P17947
Target
- Target
- Human SPI1 / PU.1
- Antigen Species
- Human
- Epitope
- This antibody has been extensively validated, including cross-reactivity testing against 19,000 full-length proteins by HuProt(TM) microarray analysis.
- Gene Name
- SPI1
Antibody
- Host Species
- Mouse
- Clonality
- Monoclonal
- Clone
- PU1/2146
- Isotype
- IgG2b
- Light Chain
- Kappa Light Chain
- Origin Species
- Human
Immunogen
- Immunogen
- A portion of amino acids 16-170 from the human protein was used as the immunogen for the PU.1 antibody.
- Immunogen Type
- Peptide
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 1X PBS
- Concentration
- 1 mg/mL
- Preservative
- No
- Purification Method
- Protein G Affinity
Storage & Handling
- Storage Conditions
- Aliquot and store at -20°C.
- Recommended Storage Buffer
- 1X PBS