Western blot analysis of Human, Mouse brain lysate showing detection of ~16 kDa Alpha Synuclein pSer129 protein using Rabbit Anti-Alpha Synuclein pSer129 Polyclonal Antibody. Lane 1: Molecular Weight Ladder (MW). Lane 2: Human brain lysate. Lane 3: Mouse brain lysate. Lane 4: Alpha Synuclein Monomer (0.5 µg). Load: 15 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-Alpha Synuclein pSer129 Polyclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~16 kDa. Other Band(s): 100, 75, 45, 30,16 kDa.
SNCA / Alpha-Synuclein Antibody (phospho-Ser129)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunofluorescence, Immunohistochemistry (general), Western Blot |
| Reactivity: | Human, Mouse, Rat |
| Format: | Liquid |
$507.00each
Catalog Number: LS-C773061-100
Catalog Number: LS-C773061
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- Description
- Alpha-Synuclein antibody LS-C773061 is an unconjugated rabbit polyclonal antibody to Alpha-Synuclein (SNCA) (pSer129) from human. It is reactive with human, mouse and rat. Validated for ELISA, IF, IHC and WB.
- Application
- ELISA, IF, IHC, WB
- Reactivity
- Hu, Ms, Rt
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- Alpha-synuclein | PARK4 | PD1 | SNCA | Synuclein alpha-140 | NACP | PARK1 | aSynuclein | a-Synuclein
- Recommended Dilution: IF/ICC
- 1:500
- Recommended Dilution: WB
- 1:1000
- UniProt Number
- P37840
- NCBI GenBank
- NM_000345 | NP_000336.1
- SwissProt
- P37840
Target
- Target
- Human SNCA / Alpha-Synuclein
- Antigen Species
- Human
- Epitope
- Binds to phosphorylated serine 129 on alpha synuclein. Does not detect unphosphorylated serine 129 alpha synuclein. Detects bands at 100, 75, 45, 15 kDa. Bands above 15 kDa are oligomers.
- Gene Name
- SNCA
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Origin Species
- Human
Immunogen
- Immunogen
- Synthetic peptide of Human Alpha Synuclein pSer129 (40-140 aa), conjugated to Keyhole Limpet Haemocyanin (KLH).
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, pH 7.4, 0.09% Sodium Azide, 50% Glycerol
- Concentration
- 0.5 mg/mL
- Volume
- 100 Microliter
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store at -20°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- PBS