Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Alpha Synuclein Monoclonal Antibody, Clone 4F1. Tissue: Primary hippocampal neurons treated with active Alpha Synuclein Protein Aggregate (SPR-322) at 4 µg/ml to induce fibrils. Species: Rat. Fixation: 4% paraformaldehyde. Primary Antibody: Mouse Anti-Alpha Synuclein Monoclonal Antibody at 1:200 for 24 hours at 4°C. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:700 for 1 hour at RT. Counterstain: Guinea Pig Anti-NeuN (red) neuronal marker (Donkey Anti-Guinea Pig Alexa Fluor 647 1:700); DAPI (blue) nuclear stain at 1:6000, 1:3000 for 60 min at RT, 5 min at RT. Magnification: 20X. (A) DAPI (blue) nuclear stain. (B) NeuN neuronal marker (red). (C) Alpha Synuclein Antibody. (D) Composite.
SNCA / Alpha-Synuclein Antibody (clone 4F1, FITC)
| Antibody: | Mouse Monoclonal |
| Applications: | Dot Blot, Enzyme-Linked Immunosorbent Assay, Immunofluorescence, Immunohistochemistry (general), Western Blot |
| Reactivity: | Human, Mouse, Rat |
| Format: | Liquid |
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- Description
- Alpha-Synuclein antibody LS-C772995 is an FITC-conjugated mouse monoclonal antibody to Alpha-Synuclein (SNCA) from human. It is reactive with human, mouse and rat. Validated for DB, ELISA, IF, IHC and WB.
- Application
- DB, ELISA, IF, IHC, WB
- Reactivity
- Hu, Ms, Rt
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Alternative Names
- Alpha-synuclein | PARK4 | PD1 | SNCA | Synuclein alpha-140 | NACP | PARK1 | aSynuclein | a-Synuclein
- Recommended Dilution: ELISA
- 1:1000
- Recommended Dilution: IF/ICC
- 1:100
- Recommended Dilution: IHC
- 1:200
- Recommended Dilution: Other
- Dot Blot: 1:1000
- Recommended Dilution: WB
- 1:1000
- UniProt Number
- P37840
- NCBI GenBank
- NM_000345 | NP_000336.1
- SwissProt
- P37840
Target
- Target
- Human SNCA / Alpha-Synuclein
- Antigen Species
- Human
- Epitope
- Binds multiple linear epitopes within human alpha synuclein: 21-70 aa, 61-90 aa, and 101-140 aa, determined using Dot blot and ELISA with corresponding peptides. Detects ~14 kDa band corresponding to Alpha synuclein. Band at ~40 kDa is a trimer.
- Gene Name
- SNCA
Antibody
- Host Species
- Mouse
- Clonality
- Monoclonal
- Clone
- 4F1
- Isotype
- IgG1
- Origin Species
- Human
Immunogen
- Immunogen
- Mouse alpha synuclein aggregate
Format
- Conjugate/Label
- Fluorescein Isothiocyanate
- Format
- Liquid
- Formulation
- PBS, pH 7.4, 0.09% Sodium Azide, 50% Glycerol
- Concentration
- 1 mg/mL
- Preservative
- Yes
- Purification Method
- Protein G Affinity
Storage & Handling
- Storage Conditions
- Store at -20°C.
- Recommended Storage Buffer
- PBS