SMAD2 Antibody
Catalog Number: LS-C393630-100
100 µL / $399.00
Antibody: Mouse Monoclonal
Applications: Immunocytochemistry, Western Blot
Reactivity: Human
Format: Liquid
Fluorescent confocal image of SY5Y cells stained with SMAD2 antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated SMAD2 primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). Note the highly specific localization of the SMAD2 mainly to the nucleus.
SMAD2
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Flow Cytometry, Immunofluorescence, Immunohistochemistry (general), Western Blot |
| Reactivity: | Human, Mouse |
| Format: | Liquid |
$401.00each
Catalog Number: LS-C100546-400
Catalog Number: LS-C100546
For bulk orders or custom formulations:
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- Description
- SMAD2 antibody LS-C100546 is an unconjugated rabbit polyclonal antibody to SMAD2 (Ser118) from human. It is reactive with human and mouse. Validated for Flow, IF and WB.
- Application
- FC, IF, IHC, WB
- Reactivity
- Hu, Ms
- Predicted Reactivity
- Bov, Ck, Dros, Por, Rt, Zf
- Intended Use
- RUO
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- HMAD-2 | HSMAD2 | JV18-1 | Mad protein homolog smad2 | MADR2 | MADH2 | SMAD 2 | MAD homolog 2 | Mad-related protein 2 | SMAD2 | JV18 | Mother against DPP homolog 2 | Mothers against DPP homolog 2 | Sma- and Mad-related protein 2 | SMAD family member 2
- Recommended Dilution: FC
- 1:10 - 1:50
- Recommended Dilution: IF/ICC
- 1:100
- Recommended Dilution: WB
- 1:1000
- UniProt Number
- Q15796
- NCBI GenBank
- NM_005901, NP_005892.1
- SwissProt
- Q15796
Target
- Target
- Human SMAD2
- Antigen Species
- Hu
- Epitope
- This SMAD2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 97-125 amino acids from human SMAD2.
- Gene Name
- SMAD2
Antibody
- Host Species
- Rb
- Clonality
- Polyclonal
Immunogen
- Immunogen Type
- Pep
Format
- Conjugate/Label
- Unconj
- Format
- Liquid
- Formulation
- PBS, 0.09% Sodium Azide
- Volume
- 400 Microliter
- Preservative
- Yes
- Purification Method
- Prot A
Storage & Handling
- Storage Conditions
- Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
- Recommended Storage Buffer
- PBS
