Western Blot of rabbit anti-Smad2 pS465pS467 antibody. Lane 1: MDA-MB-231 cells. Lane 2: MDA-MB-231 cells treated with TGF-β1 for 1h. Load: 20 µg per lane. Primary antibody: Smad2pS465pS467 antibody at 1:1000 for overnight at 4°C. Secondary antibody: rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO/TBST overnight at 4°C. Predicted/Observed size: 52.3 kDa for Smad2pS465pS467. Other band(s): ~85kDa non-specific band.

SMAD2 Antibody (phospho-Ser465/467)

Primary AntibodyLSBio Guarantee

Antibody:	Rabbit Polyclonal
Applications:	Enzyme-Linked Immunosorbent Assay, Western Blot
Reactivity:	Human
Format:	Liquid

$311.00each

Catalog Number: LS-C745495-25

Unit Size

Quantity

For bulk orders or custom formulations:

Overview

Description: SMAD2 antibody LS-C745495 is an unconjugated rabbit polyclonal antibody to human SMAD2 (pSer465/467). Validated for ELISA and WB.
Application: ELISA, WB
Reactivity: Hu
Predicted Reactivity: Bovine, Mouse, Non-Human Primate, Rat
Intended Use: Research Use Only
Guarantees: This antibody carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names: HMAD-2 | HSMAD2 | JV18-1 | Mad protein homolog smad2 | MADR2 | MADH2 | SMAD 2 | MAD homolog 2 | Mad-related protein 2 | SMAD2 | JV18 | Mother against DPP homolog 2 | Mothers against DPP homolog 2 | Sma- and Mad-related protein 2 | SMAD family member 2
Recommended Dilution: ELISA: 1:10000
Recommended Dilution: WB: 1:1000 - 1:3000
UniProt Number: Q15796
NCBI GenBank: NM_005901 | NP_005892.1
SwissProt: Q15796

Target

Target: Human SMAD2
Antigen Species: Human
Epitope: Anti-SMAD2 pS465 pS467 antibody is directed against the phosphorylated form of human Smad2 protein at the pS465 and pS467 residues. Reactivity occurs against human SMAD2. Reactivity with non-phosphorylated human Smad2 is minimal by ELISA and western blot. A BLAST analysis was used to suggest cross-reactivity with Smad2 protein from human, mouse, rat, orangutan, and bovine based on 100% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.
Gene Name: SMAD2

Antibody

Host Species: Rabbit
Clonality: Polyclonal
Isotype: IgG
Origin Species: Human

Immunogen

Immunogen: SMAD2 pS465 pS467 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a C-terminal of human SMAD2 protein.
Immunogen Type: Synthetic Peptide (Linear)

Format

Conjugate/Label: Unconjugated
Format: Liquid
Formulation: 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration: 0.94 mg/mL
Volume: 25 Microliter
Preservative: Yes
Purification Method: Affinity Purified

Storage & Handling

Storage Conditions: Store vial at 4°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
Recommended Storage Buffer: 0.02 M Potassium Phosphate|0.15 M NaCl