Western blot analysis of extracts from COS7 cells treated (lane 1) and untreated (lane 2) with UV (15mins), and Jurkat cells treated with EGF (200ng/ml, 30mins) (lane 3), using Smad2/3 (Ab-8) antibody.

SMAD2+3 Antibody (Ab-8)

Primary AntibodyLSBio Guarantee

Antibody:	Rabbit Polyclonal
Applications:	Enzyme-Linked Immunosorbent Assay, Immunofluorescence, Western Blot
Reactivity:	Human, Mouse, Rat
Format:	Liquid

$365.00each

Catalog Number: LS-C826639-100

Unit Size

Quantity

For bulk orders or custom formulations:

Overview

Description: SMAD2+3 antibody LS-C826639 is an unconjugated rabbit polyclonal antibody to SMAD2+3 (Ab-8) from human. It is reactive with human, mouse and rat. Validated for ELISA, IF and WB.
Application: ELISA, IF, WB
Reactivity: Hu, Ms, Rt
Intended Use: Research Use Only
Guarantees: This antibody carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Polyclonal Antibodies
Recommended Dilution: ELISA: 1:10000
Recommended Dilution: WB: 1:500 - 1:1000

Target

Target: Human SMAD2+3
Antigen Species: Human
Epitope: Smad2/3 (Ab-8) Antibody detects endogenous levels of total Smad2/3 protein.

Antibody

Host Species: Rabbit
Clonality: Polyclonal
Isotype: IgG

Immunogen

Immunogen: The antiserum was produced against synthesized non-phosphopeptide derived from humanSmad2/3 around the phosphorylation site of threonine 8 (P-F-TP-P-P).
Immunogen Type: Synthetic Peptide (Linear)

Format

Conjugate/Label: Unconjugated
Format: Liquid
Formulation: PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration: 1 mg/mL
Preservative: Yes
Purification Method: Affinity Purified