Western blot analysis of extracts of various cell lines, using SLC7A1 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 60s.
SLC7A1 / CAT1 Antibody
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Immunohistochemistry (general), Western Blot |
| Reactivity: | Human, Mouse, Rat |
| Format: | Liquid |
$281.00each
Catalog Number: LS-C749764-20
Catalog Number: LS-C749764
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- Description
- CAT1 antibody LS-C749764 is an unconjugated rabbit polyclonal antibody to CAT1 (SLC7A1) from human. It is reactive with human, mouse and rat. Validated for WB. Cited in 1 publication.
- Application
- IHC, WB
- Reactivity
- Hu, Ms, Rt
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- ATRC1 | Atrc-1 | CAT1 | CAT-1 | ERR | HCAT1 | Rec-1 | REC1L | Ecotropic retroviral receptor | SLC7A1
- Recommended Dilution: WB
- 1:500 - 1:2000
- UniProt Number
- P30825
- NCBI GenBank
- NM_003045 | NP_003036.1
- SwissProt
- P30825
Target
- Target
- Human SLC7A1 / CAT1
- Antigen Species
- Human
- Gene Name
- SLC7A1
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Human
Immunogen
- Immunogen
- A synthetic peptide of human SLC7A1
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol
- Concentration
- 5.25 mg/mL
- Volume
- 20 Microliter
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store at -20°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- PBS
Loss of the mitochondrial protein Abcb10 results in altered arginine metabolism in MEL and K562 cells and nutrient stress signaling through ATF4. Marisa Miljkovic, Alexandra Seguin, Xuan Jia, James E Cox, Jonathan Leon Catrow, Hector Bergonia, John D Phillips, W Zac Stephens, Diane M Ward. The Journal of biological chemistry. 2023 July;299:104877. PubMed: 37269954 PMC: PMC10316008