Confocal immunofluorescence imaging of dopamine transporter (DAT) using the monoclonal antibody purified from mouse hybridoma clone 6V-23-23. Lewis Lung Carcinoma Porcine Kidney cells engineered to stably express the rat dopamine transporter were fixed by standard methods and stained for DAT using the monoclonal antibody from clone 6V-23-23 and FITC-conjugated secondary antibody. Staining is negligible in cells treated without primary antibody (not shown).
SLC6A3 / Dopamine Transporter Antibody (clone 6V-23-23)
Primary AntibodyLSBio Guarantee
| Antibody: | Mouse Monoclonal |
| Applications: | Immunocytochemistry, Immunofluorescence, Western Blot |
| Reactivity: | Mouse, Rat |
| Format: | Liquid |
$492.00each
Catalog Number: LS-C827962-0.1
Catalog Number: LS-C827962
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- Description
- Dopamine Transporter antibody LS-C827962 is an unconjugated mouse monoclonal antibody to Dopamine Transporter (SLC6A3) from rat. It is reactive with mouse and rat. Validated for ICC, IF and WB.
- Application
- ICC, IF, WB
- Reactivity
- Ms, Rt
- Nonreactive Species
- Hu
- Intended Use
- RUO
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Alternative Names
- DA transporter | DAT1 | DAT | SLC6A3
- Recommended Dilution: IF/ICC
- 1:200
- Recommended Dilution: WB
- 1:1000
- UniProt Number
- Q01959
- NCBI GenBank
- NM_001044, NP_001035.1
- SwissProt
- Q01959
Target
- Target
- Rat SLC6A3 / Dopamine Transporter
- Antigen Species
- Rt
- Gene Name
- SLC6A3
Antibody
- Host Species
- Ms
- Clonality
- Monoclonal
- Clone
- 6V-23-23
- Isotype
- IgG1
- Origin Species
- Hu
Immunogen
- Immunogen
- Synthetic amino acid N-terminal peptide of rat dopamine transporter
- Immunogen Type
- SP-Lin
Format
- Conjugate/Label
- Unconj
- Format
- Liquid
- Formulation
- 20 mM Potassium Phosphate, pH 7.2, 150 mM NaCl, 0.1% Sodium Azide
- Concentration
- 1.62 mg/mL
- Volume
- 0.1 Milliliter
- Preservative
- Yes
- Purification Method
- Prot A
Storage & Handling
- Storage Conditions
- Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- 20 mM Potassium Phosphate|150 mM NaCl