Immunoprecipitation(IP) of SLC2A5 by using monoclonal anti-SLC2A5 antibodies (Negative control: IP without adding anti-SLC2A5 antibody.). For each experiment, 500ul of DDK tagged SLC2A5 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2 ug of anti-SLC2A5 antibody and 20ul (0.1 mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
SLC2A5 / GLUT5 Antibody (clone OTI2C4)
Primary AntibodyLSBio Guarantee
| Antibody: | Mouse Monoclonal |
| Applications: | Immunohistochemistry (general), Immunohistochemistry, Paraffin, Immunoprecipitation, Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$443.00each
Catalog Number: LS-C114643-100
Catalog Number: LS-C114643
For bulk orders or custom formulations:
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- Description
- GLUT5 antibody LS-C114643 is an unconjugated mouse monoclonal antibody to human GLUT5 (SLC2A5). Validated for IHC, IP and WB.
- Application
- IHC, IHC-P, IP, WB
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Alternative Names
- Fructose transporter | Glucose transporter 5 | GLUT-5 | GLUT5 | SLC2A5
- Recommended Dilution: IHC-P
- 1:50
- Recommended Dilution: WB
- 1:500 - 1:1000
- UniProt Number
- P22732
- NCBI GenBank
- NM_003039 | NP_003030.1
- SwissProt
- P22732
Target
- Target
- Human SLC2A5 / GLUT5
- Antigen Species
- Human
- Epitope
- Human GLUT5
- Gene Name
- SLC2A5
Antibody
- Host Species
- Mouse
- Clonality
- Monoclonal
- Clone
- OTI2C4
- Isotype
- IgG1
Immunogen
- Immunogen
- Full length human recombinant protein of human SLC2A5 (NP_003030) produced in HEK293T cell.
- Immunogen Type
- Full-Length Recombinant Protein
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol, 1% BSA
- Concentration
- 0.84 mg/mL
- Volume
- 100 Microliter
- Preservative
- Yes
- Purification Method
- Protein A/G Affinity
Storage & Handling
- Storage Conditions
- Store at -20°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- PBS