Western blot analysis of extracts of various cell lines, using HHAT antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 60s.
Skn / HHAT Antibody
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Western Blot |
| Reactivity: | Human, Mouse, Rat |
| Format: | Liquid |
$281.00each
Catalog Number: LS-C750145-20
Catalog Number: LS-C750145
For bulk orders or custom formulations:
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- Description
- HHAT antibody LS-C750145 is an unconjugated rabbit polyclonal antibody to HHAT (Skn) from human. It is reactive with human, mouse and rat. Validated for WB.
- Application
- WB
- Reactivity
- Hu, Ms, Rt
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- Gup2 | MART-2 | MART2 | Hedgehog acyltransferase | Rasp | Skinny hedgehog protein 1 | HHAT | SIT | SKI1 | Skn
- Recommended Dilution: WB
- 1:500 - 1:2000
- UniProt Number
- Q5VTY9
- NCBI GenBank
- NM_018194 | NP_060664.2
- SwissProt
- Q5VTY9
Target
- Target
- Human Skn / HHAT
- Antigen Species
- Human
- Gene Name
- HHAT
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Human
Immunogen
- Immunogen
- A synthetic peptide corresponding to a sequence within amino acids 1-100 of human HHAT (NP_060664.2). MLPRWELALYLLASLGFHFYSFYEVYKVSREHEEELDQEFELETDTLFGGLKKDATDFEWSFWMEWGKQWLVWLLLGHMVVSQMATLLARKHRPWILMLY
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol
- Volume
- 20 Microliter
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store at -20°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- PBS