SIRT1 was immunoprecipitated using: Lane A: 0.5 mg Hela Whole Cell Lysate. 4 uL anti-SIRT1 rabbit polyclonal antibody and 15 ul of 50% Protein G agarose. Primary antibody: Anti-SIRT1 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 82 kDa. Observed band size: 130 kDa.
SIRT1 / Sirtuin 1 Antibody (Preservative Free)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$309.00each
Catalog Number: LS-C818303-100
Catalog Number: LS-C818303
For bulk orders or custom formulations:
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- Description
- Sirtuin 1 antibody LS-C818303 is an unconjugated rabbit polyclonal antibody to human Sirtuin 1 (SIRT1). Validated for ELISA, ICC, IF, IP and WB.
- Application
- ELISA, ICC, IF, IP, WB
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- SIR2-like protein 1 | SIR2alpha | SIR2L1 | Sirtuin 1 | Sir2-like 1 | SIRT1 | Sirtuin type 1 | HSIR2 | HSIRT1
- Recommended Dilution: ELISA
- 1:5000 - 1:10000
- Recommended Dilution: IF/ICC
- 1:300 - 1:10000
- Recommended Dilution: WB
- 1:500 - 1:2000
- UniProt Number
- Q96EB6
- NCBI GenBank
- NM_012238 | NP_036370.2
- SwissProt
- Q96EB6
Target
- Target
- Human SIRT1 / Sirtuin 1
- Antigen Species
- Human
- Epitope
- human SIRT1
- Gene Name
- SIRT1
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Human
Immunogen
- Immunogen
- Recombinant Human SIRT1 protein
- Immunogen Type
- Recombinant Immunogen
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.2 µm filtered solution in PBS
- Volume
- 100 Microliter
- Preservative
- No
- Purification Method
- Protein A Affinity
Storage & Handling
- Storage Conditions
- Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.
- Recommended Storage Buffer
- PBS