Western blot of recombinant human sirtuins, Jurkat cells and HeLa nuclear extract. The nitrocellulose was probed with anti- SIRT1 at 1:1000. The secondary antibody was coupled to alkaline phosphatase and the blot was developed with BCIP/NBT. Arrows indicate positions of molecular weight markers. Lanes: S2, S3, S5 (SIRTs 2, 3, 5, 50 ng each); J (150,000 Jurkat cells); H (18 ug HeLa nuclear extract); S1 (SIRT1- 5 ng, 0.5 ng).
SIRT1 / Sirtuin 1 Antibody
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$532.00each
Catalog Number: LS-C343825-100
Catalog Number: LS-C343825
For bulk orders or custom formulations:
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- Description
- Sirtuin 1 antibody LS-C343825 is an unconjugated rabbit polyclonal antibody to human Sirtuin 1 (SIRT1). Validated for IHC and WB.
- Application
- IHC, IHC-P, WB
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- SIR2-like protein 1 | SIR2alpha | SIR2L1 | Sirtuin 1 | Sir2-like 1 | SIRT1 | Sirtuin type 1 | HSIR2 | HSIRT1
- Recommended Dilution: WB
- 1:1000
- UniProt Number
- Q96EB6
- NCBI GenBank
- NM_012238 | NP_036370.2
- SwissProt
- Q96EB6
Target
- Target
- Human SIRT1 / Sirtuin 1
- Antigen Species
- Human
- Gene Name
- SIRT1
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
Immunogen
- Immunogen
- Recombinant human SIRT1 (sirtuin 1).
- Immunogen Type
- Recombinant Immunogen
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, pH 7.4
- Volume
- 100 Microliter
- Preservative
- No
- Purification Method
- Protein A Affinity
Storage & Handling
- Storage Conditions
- Store at -20°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- PBS