Western Blot of rabbit anti-SIRT1 antibody. Lane 1: MW ladder (opal pre-stained). Lane 2: HCT-116 WCL. Lane 3: K-562 WCL. Lane 4: Jurkat WCL. Lane 5: A549 WCL. Lane 6: LnCap WCL. Load: 35 µg per lane. Primary antibody: SIRT1 antibody at 1:1000 for overnight at 4°C. Secondary antibody: rabbit secondary HRP antibody at 1:70,000 for 45 min at RT. Block: BlockOut overnight at 4°C. Predicted size: 82 kDa, 61 kDa for SIRT1. Observed size: ~110 kda due to complexing proteins.
SIRT1 / Sirtuin 1 Antibody (C-Terminus)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Western Blot |
| Format: | Liquid |
$579.00each
Catalog Number: LS-C745420-100
Catalog Number: LS-C745420
For bulk orders or custom formulations:
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- Description
- Sirtuin 1 antibody LS-C745420 is an unconjugated rabbit polyclonal antibody to human Sirtuin 1 (SIRT1) (C-Terminus). Validated for ELISA and WB.
- Application
- ELISA, WB
- Predicted Reactivity
- Hu, Ms, Rt
- Intended Use
- RUO
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- SIR2-like protein 1 | SIR2alpha | SIR2L1 | Sirtuin 1 | Sir2-like 1 | SIRT1 | Sirtuin type 1 | HSIR2 | HSIRT1
- Recommended Dilution: ELISA
- 5 ug/ml
- Recommended Dilution: WB
- 1 µg/ml
- UniProt Number
- Q96EB6
- NCBI GenBank
- NM_012238, NP_036370.2
- SwissProt
- Q96EB6
Target
- Target
- Human SIRT1 / Sirtuin 1
- Antigen Species
- Hu
- Epitope
- A BLAST analysis used to suggest reactivity with this protein from human, mouse, rat based on 100% homology for the immunizing sequence.
- Gene Name
- SIRT1
Antibody
- Host Species
- Rb
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Hu
Immunogen
- Immunogen
- Affinity purified Anti-SIRT1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide near the c-terminal of human SIRT1 protein.
- Immunogen Type
- SP-Lin
Format
- Conjugate/Label
- Unconj
- Format
- Liquid
- Formulation
- 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
- Concentration
- 1.07 mg/mL
- Preservative
- Yes
- Purification Method
- AP
Storage & Handling
- Storage Conditions
- Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl