Immunofluorescent analysis of CD172a staining in THP1 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
SIRPA / CD172a Antibody (C-Terminus)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Immunocytochemistry, Immunofluorescence, Immunohistochemistry (general), Immunoprecipitation, Western Blot |
| Reactivity: | Human, Non-Human Primate |
| Format: | Liquid |
$261.00each
Catalog Number: LS-C353657-30
Catalog Number: LS-C353657
For bulk orders or custom formulations:
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- Description
- CD172a antibody LS-C353657 is an unconjugated rabbit polyclonal antibody to CD172a (SIRPA) (C-Terminus) from human. It is reactive with human and monkey. Validated for IF and WB.
- Application
- ICC, IF, IHC, IP, WB
- Reactivity
- Hu, NHP
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- CD172A | CD172a antigen | Inhibitory receptors shps-1 | Inhibitory receptor SHPS-1 | Macrophage fusion receptor | MyD-1 antigen | p84 | SHPS-1 | SHPS1 | SIRP | Sirp-alpha-1 | Sirp-alpha-3 | Sirp-alpha1 | SIRPA | MYD1 | SIRPalpha | SIRPalpha2 | BIT | MFR | MYD-1 | PTPNS1 | SHP substrate 1 | Sirp-alpha-2 | CD172
- Recommended Dilution: IF/ICC
- 1:100 - 1:500
- Recommended Dilution: IP
- 1:10 - 1:100
- Recommended Dilution: WB
- 1:500 - 1:1000
- UniProt Number
- P78324
- NCBI GenBank
- NM_080792 | NP_542970.1
- SwissProt
- P78324
Target
- Target
- Human SIRPA / CD172a
- Antigen Species
- Human
- Epitope
- Recognizes endogenous levels of CD172a protein.
- Gene Name
- SIRPA
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
Immunogen
- Immunogen
- KLH-conjugated synthetic peptide encompassing a sequence within the C-Terminus of human CD172a.
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol
- Volume
- 30 Microliter
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store at -20°C. Aliquot to avoid freeze/thaw cycles.
- Recommended Storage Buffer
- PBS