Human peripheral blood monocytes stained with Mouse anti-Human Siglec-5/Siglec-14:FITC This image was taken for the unconjugated form of this product. Other forms have not been tested.
SIGLEC5 + SIGLEC14 Antibody (clone 1A5, FITC)
Primary AntibodyLSBio Guarantee
| Antibody: | Mouse Monoclonal |
| Applications: | Flow Cytometry, Immunohistochemistry (general) |
| Reactivity: | Human |
| Format: | Liquid |
$335.00each
Catalog Number: LS-C187567-0.1
Catalog Number: LS-C187567
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- SIGLEC5 + SIGLEC14 antibody LS-C187567 is an FITC-conjugated mouse monoclonal antibody to human SIGLEC5 + SIGLEC14. Validated for Flow.
- Application
- FC, IHC
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
Target
- Target
- Human SIGLEC5 + SIGLEC14
- Antigen Species
- Human
- Epitope
- Clone 1A5 recognizes human Siglec-5 (Sialic acid-binding Ig-like lectin 5), otherwise known as CD170, a novel sialic-acid-binding Ig-like lectin, and member of the Ig superfamily, expressed by dendritic cells (DCs), activated macrophages, neutrophils, and cells of the monocyte/myeloid lineage. Clone 1A5, is one of several Siglec-5 antibodies which also recognizes human Siglec-14 (Sialic acid-binding Ig-like lectin 14) see for details. Siglec-14 shares an almost identical sequence with Siglec-5 within the first two Ig-like domains, indicating partial gene conversion between these two Siglecs, also evident in other primate species. Siglec-5 is highly related to the myelomonocytic-derived adhesion molecule CD33 (Siglec-3), and mediates sialic-acid dependent binding to cells, as well as acting as an inhibitory receptor in the down-regulation of cell activation. Structurally, Siglec-5 contains an immunoreceptor tyrosine-based inhibitor motif (ITIM), which plays a part in the modulation of cellular responses, and when phosphorylated, can bind to the SH2 domain of several SH2-containing phosphatases. Siglec-14 is a putative sialic-acid binding adhesion molecule, and member of the Ig superfamily, predominantly expressed in hematopoietic tissues, which has been shown to associate with the activating adapter protein DAP12. Clone 1A5 has been show to cross react with Chimpanzee see for details.
Antibody
- Host Species
- Mouse
- Clonality
- Monoclonal
- Clone
- 1A5
- Isotype
- IgG1
Immunogen
- Immunogen
- Siglec-5-Fc protein, consisting of the full-length extracellular region of human Siglec-5, fused with the Fc region of human IgG1.
Format
- Conjugate/Label
- Fluorescein Isothiocyanate
- Format
- Liquid
- Formulation
- PBS, 0.09% Sodium Azide, 1% BSA
- Concentration
- 0.1 mg/mL
- Preservative
- Yes
- Purification Method
- Protein G Affinity
Storage & Handling
- Storage Conditions
- Store at 4°C or at -20°C. Store undiluted. Avoid freeze-thaw cycles. Protect from light. Microcentrifugation recommended if solution contains precipitate.
- Recommended Storage Buffer
- PBS