SIAH 1/2 Antibody (8G7H12) - Anti-SINA/SIAH monoclonal antibodies recognize both Drosophila SINA and human SIAH. There are two SINA-like E3 ligases, SINA and SINAH, in Drosophila. SINA monoclonal antibody 8G7H12 is highly specific in recognizing both Drosophila SINA and human SIAH proteins but not Drosophila SINAH. Coomassie blue staining patterns of both the wild-type (WT) and dominant negative (PD) forms of affinity-purified recombinant GST-SINA/SINAH/SIAH fusion proteins are shown in the top panel. Protein standards were included and are shown in kD. Cross-reactivity of SINA monoclonal antibody 8G7H12 against Drosophila SINA and SINAH, and human hSIAH-1 and hSIAH-2 proteins were determined. Anti-SINA-C-terminal antibody 8G7H12 recognizes both human SIAH-1 and SIAH-2 protein.