LSBio
Sheep Anti-Mouse IgG Secondary Antibody (HRP)

MOUSE IgG (H&L) Antibody Peroxidase Conjugated. Western Blot of Peroxidase conjugated Sheep anti-Mouse IgG antibody. Lane 1: Mouse IgG. Load: 100 ng per lane. Primary antibody: none. Secondary antibody: Peroxidase Mouse secondary antibody at 1:1000 for 60 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 55 and 28 kD, 55 and 28 kD for Mouse IgG. Other band(s): none. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Sheep Anti-Mouse IgG Secondary Antibody (HRP)

Secondary AntibodyLSBio Guarantee
Antibody:Sheep Polyclonal
Applications:Dot Blot, Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Other, Western Blot
Reactivity:Mouse
Format:Lyophilized
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$340.00each
Catalog Number: LS-C60831-2

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Overview

Description
Mouse IgG antibody LS-C60831 is an HRP-conjugated sheep polyclonal antibody. Validated for DB, ELISA, EM, IHC and WB.
Application
DB, ELISA, IHC, Other, WB
Reactivity
Ms
Intended Use
RUO
Guarantees
This antibody carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Secondary Antibodies
Recommended Dilution: ELISA
1:100000 - 1:200000
Recommended Dilution: IHC
1:500 - 1:2500
Recommended Dilution: WB
1:1000 - 1:5000

Target

Target
Mouse IgG
Target Heavy Chain
IgG
Antigen Species
Ms
Epitope
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Sheep Serum, Mouse IgG and Mouse Serum.

Antibody

Host Species
Sh
Clonality
Polyclonal
Isotype
IgG

Immunogen

Immunogen
Anti-Mouse IgG [H&L] was produced by repeated immunization with Mouse IgG whole molecule in sheep.
Immunogen Type
Native

Format

Conjugate/Label
HRP
Format
Lyophilized
Formulation
Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Gentamicin. Do NOT add Sodium Azide
Concentration
2 mg/mL
Preservative
Yes
Purification Method
AP

Storage & Handling

Storage Conditions
Store at 4°C.
Recommended Storage Buffer
0.02 M Potassium Phosphate|0.15 M NaCl

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.