Anti-Human IgA Secondary Antibody
Catalog Number: LS-C758146-1
1 mg / $306.00
Antibody: Human Polyclonal
Applications: Enzyme-Linked Immunosorbent Assay, Western Blot
Reactivity: Human
Format: Liquid
ELISA results of purified Sheep Anti-HUMAN IgG Biotin Conjugated Antibody tested against purified Human IgG. Each well was coated in duplicate with 1.0 µg of human IgG (green line). The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Blocking buffer MB-Streptavidin HRP conjugate 1:10,000 and TMB-1000 substrate.
Sheep Anti-Human IgG Secondary Antibody (Biotin)
Secondary AntibodyLSBio Guarantee
| Antibody: | Sheep Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunoblot, Immunohistochemistry (general), Western Blot |
| Reactivity: | Human |
| Format: | Lyophilized |
$334.00each
Catalog Number: LS-C60639-2
Catalog Number: LS-C60639
For bulk orders or custom formulations:
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- Description
- Human IgG antibody LS-C60639 is a biotin-conjugated sheep polyclonal antibody. Validated for ELISA, IHC and WB.
- Application
- ELISA, IB, IHC, WB
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Secondary Antibodies
- Recommended Dilution: ELISA
- 1:20000 - 1:100000
- Recommended Dilution: IHC
- 1:1000 - 1:5000
- Recommended Dilution: WB
- 1:2000 - 1:10000
Target
- Target
- Human IgG
- Antigen Species
- Human
- Epitope
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Sheep Serum, Human IgG and Human Serum.
Antibody
- Host Species
- Sheep
- Clonality
- Polyclonal
- Isotype
- IgG
Immunogen
- Immunogen
- Human IgG whole molecule
- Immunogen Type
- Native Protein (Purified from Natural Source)
Format
- Conjugate/Label
- Biotin
- Format
- Lyophilized
- Formulation
- Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.01 M Sodium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles. Store undiluted.
- Recommended Storage Buffer
- 0.01 M Sodium Phosphate|0.15 M NaCl