LSBio
SHANK3 Antibody (clone S367-51, Atto 594)

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-SHANK1/SHANK3 Monoclonal Antibody, Clone S367-51. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-SHANK1/SHANK3 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Cytoplasm, Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) SHANK1/SHANK3 Antibody. (D) Composite.

SHANK3 Antibody (clone S367-51, Atto 594)

Primary AntibodyLSBio Guarantee
Antibody:Mouse Monoclonal
Applications:Immunofluorescence, Immunohistochemistry (general), Western Blot
Reactivity:Human, Mouse, Rat
Format:Liquid
See all Human SHANK3 antibodies →
$489.00each
Catalog Number: LS-C773380-100

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Overview

Description
SHANK3 antibody LS-C773380 is an Atto 594-conjugated mouse monoclonal antibody to SHANK3 from human. It is reactive with human, mouse and rat. Validated for IF, IHC and WB.
Application
IF, IHC, WB
Reactivity
Hu, Ms, Rt
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Monoclonal Antibodies
Alternative Names
DEL22q13.3 | KIAA1650 | SHANK3 | SPANK-2 | PROSAP2 | PSAP2 | SCZD15
Recommended Dilution: IF/ICC
1:100
Recommended Dilution: WB
1:1000
UniProt Number
Q9BYB0
NCBI GenBank
NP_001073889.1
SwissProt
Q9BYB0

Target

Target
Human SHANK3
Antigen Species
Human
Epitope
Detects ~190 kDa. Cross-reacts with SHANK1. Does not cross-react with SHANK2.
Gene Name
SHANK3

Antibody

Host Species
Mouse
Clonality
Monoclonal
Clone
S367-51
Isotype
IgG2a
Origin Species
Human

Immunogen

Immunogen
Fusion protein amino acids 538-626 (SH3 domain) of rat SHANK3.
Immunogen Type
Fusion Protein (e.g., GST, MBP tag)

Format

Conjugate/Label
Atto 594
Format
Liquid
Formulation
PBS, pH 7.4, 0.1% Sodium Azide, 50% Glycerol
Concentration
1 mg/mL
Preservative
Yes
Purification Method
Protein G Affinity

Storage & Handling

Storage Conditions
Store at -20°C.
Recommended Storage Buffer
PBS

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.