Western blot analysis of SFRP4 expression in A549 whole cell lysates (lane 1) and SW620 whole cell lysates (lane 2). SFRP4 at 49 kD was detected using rabbit anti- SFRP4 Antigen Affinity purified polyclonal antibody at 0.5 ug/mL. The blot was developed using chemiluminescence (ECL) method.

SFRP4 Antibody (aa22-303)

Primary AntibodyLSBio Guarantee

Antibody:	Rabbit Polyclonal
Applications:	Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot
Reactivity:	Human
Format:	Lyophilized

$480.00each

Catalog Number: LS-C408100-100

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Quantity

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Overview

Description: SFRP4 antibody LS-C408100 is an unconjugated rabbit polyclonal antibody to human SFRP4 (aa22-303). Validated for ELISA, IHC and WB.
Application: ELISA, IHC, IHC-P, WB
Reactivity: Hu
Intended Use: Research Use Only
Guarantees: This antibody carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names: FRPHE | SFRP-4 | SFRP4 | FRP-4
Recommended Dilution: ELISA: 0.1 - 0.5 ug/ml
UniProt Number: Q6FHJ7
NCBI GenBank: NM_003014 | NP_003005.2
SwissProt: Q6FHJ7

Target

Target: Human SFRP4
Antigen Species: Human
Epitope: Expressed in mesenchymal cells. Highly expressed in the stroma of proliferative endometrium. Expressed in cardiomyocytes. Shows moderate to strong expression in ovarian tumors with expression increasing as the tumor stage increases. In ovarian tumors, expression levels are inversely correlated with expression of CTNNB1 (at protein level).
Gene Name: SFRP4

Antibody

Host Species: Rabbit
Clonality: Polyclonal

Immunogen

Immunogen: E. coli-derived human SFRP4 recombinant protein (Position: A22-K303). Human SFRP4 shares 96.8% and 96.1% amino acid (aa) sequence identity with mouse and rat SFRP4, respectively.
Immunogen Type: Recombinant Immunogen

Format

Conjugate/Label: Unconjugated
Format: Lyophilized
Formulation: Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg sodium azide.
Preservative: Yes
Purification Method: Affinity Purified

Storage & Handling

Storage Conditions: At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Recommended Storage Buffer: NaCl