Immunofluorescence staining of mSFN in Raw264.7 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Mouse mSFN polyclonal antibody (dilution ratio 1:200) at 4°C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Cytoplasm.
SFN / Stratifin / 14-3-3 Sigma Antibody (Preservative Free)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Paraffin, Immunoprecipitation, Western Blot |
| Reactivity: | Mouse |
| Format: | Liquid |
$309.00each
Catalog Number: LS-C818411-100
Catalog Number: LS-C818411
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- Description
- 14-3-3 Sigma antibody LS-C818411 is an unconjugated rabbit polyclonal antibody to mouse 14-3-3 Sigma (SFN / Stratifin). Validated for ELISA, ICC, IF, IHC, IP and WB.
- Application
- ELISA, ICC, IF, IHC-P, IP, WB
- Reactivity
- Ms
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- 14-3-3 sigma | 14-3-3 protein sigma | HME1 | SFN | Stratifin | YWHAS
- Recommended Dilution: ELISA
- 1:5000 - 1:10000
- Recommended Dilution: IF/ICC
- 1:100 - 1:500
- Recommended Dilution: IHC-P
- 1:2000 - 1:20000
- Recommended Dilution: WB
- 1:500 - 1:2000
- UniProt Number
- P31947
- NCBI GenBank
- NM_006142 | NP_006133.1
- SwissProt
- P31947
Target
- Target
- Mouse SFN / Stratifin / 14-3-3 Sigma
- Antigen Species
- Mouse
- Epitope
- Mouse 14-3-3 sigma
- Gene Name
- SFN
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Human
Immunogen
- Immunogen
- Recombinant Mouse 14-3-3 sigma/SFN Protein
- Immunogen Type
- Recombinant Immunogen
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.2 µm filtered solution in PBS
- Volume
- 100 Microliter
- Preservative
- No
- Purification Method
- Protein A Affinity
Storage & Handling
- Storage Conditions
- Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.
- Recommended Storage Buffer
- PBS