Western Blot of Goat Anti-ALPHA 1 ANTI TRYPSIN Antibody Lane 1: reduced Alpha-1anti-Trypsin. Lane 2: none. Load: ~1ug per lane. Primary antibody: Alpha-1-Anti-Trypsin antibody at 1:3000 for overnight at 4°C. Secondary antibody: Dylight 488 conjugated Donkey anti goat secondary antibody at 1:10,000 for 1.5 hr at RT. Block: MB-070 buffer overnight at 4°C. Predicted/Observed size: 46.7kDa, ~55kDa for Alpha-1-AntiTrypsin. Other band(s): none.
SERPINA1 / Alpha 1 Antitrypsin Antibody (Lyophilized, HRP)
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| Antibody: | Goat Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Western Blot |
| Reactivity: | Human |
| Format: | Lyophilized |
$564.00each
Catalog Number: LS-C744708-100
Catalog Number: LS-C744708
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- Description
- Alpha 1 Antitrypsin antibody LS-C744708 is an HRP-conjugated goat polyclonal antibody to human Alpha 1 Antitrypsin (SERPINA1). Validated for ELISA, IHC and WB.
- Application
- ELISA, IHC, WB
- Reactivity
- Hu
- Intended Use
- RUO
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- A1A | A1AT | AAT | Alpha 1 Antitrypsin | Alpha-1 protease inhibitor | Alpha-1-antiproteinase | Alpha-1-antitrypsin | Alpha1AT | PRO2275 | Alpha-1-antitrypsin null | SERPINA1 | PI1 | Serpin A1 | Alpha 1 AT
- Recommended Dilution: ELISA
- 1:1000 - 1:5000
- Recommended Dilution: IHC
- 1:200 - 1:1000
- Recommended Dilution: WB
- 1:500 - 1:2500
- UniProt Number
- P01009
- NCBI GenBank
- AAB59495.1, K02212
- SwissProt
- P01009
Target
- Target
- Human SERPINA1 / Alpha 1 Antitrypsin
- Antigen Species
- Hu
- Epitope
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified a1-Anti-Trypsin [Human Plasma]. Cross reactivity against a1-Anti-Trypsin from other sources is unknown.
- Gene Name
- SERPINA1
Antibody
- Host Species
- Gt
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Hu
Immunogen
- Immunogen
- Alpha-1-Anti-Trypsin Antibody was produced by repeated immunizations with native a1-Anti-Trypsin protein from human plasma.
- Immunogen Type
- Native
Format
- Conjugate/Label
- HRP
- Format
- Lyophilized
- Formulation
- Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Gentamicin. Do NOT add Sodium Azide
- Concentration
- 1 mg/mL
- Preservative
- Yes
- Purification Method
- IEX
Storage & Handling
- Storage Conditions
- Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl