Alpha-1-Anti-Trypsin Polyclonal Antibody-Multiplex Fluorescent Western blot. Goat-anti-Alpha-1-Anti-Trypsin (Anti-ALPHA-1-ANTI-TRYPSIN (Human Plasma) (GOAT) Antibody lot 5842, red), Rabbit anti-Transferrin (LS-C59393 lot 3033), and Mouse-a-GST (LS-C19065 lot 24882) were used in a multiplex system to detect target proteins under reducing (R) conditions (+4% BME) in albumin depleted human serum with 320 ng of added GST. Sample was run by SDS-PAGE, transferred to 0.2 um PVDF using the Bio-Rad Trans-Blot Turbo and blocked in 2.5% Blotto, 2.5% BSA, 0.02% Tween over night at 4?. Membrane was probed with three primary antibodies at 1:1000 dilution (in MB-070 over night at 4?). Detection shown was using DyLight549 Donkey anti-Rabbit IgG (lot 21100, shown as green) DyLight 488 Donkey anti-Mouse IgG (blue), and DyLight 649 Donkey anti-Goat IgG (red) at 1:10000 (in MB-070 30 min RT). Blots were washed, rinsed in methanol, dried and Images were collected using the Bio-Rad VersaDoc System.