Anti-SPS1 Antibody - Western Blot. Western blot of anti-SPS1 antibody shows detection of endogenous SPS1 in NIH3T3 cell. No signal is seen in lysates from cells after pre-treatment with SPS1 siRNA. Endogenous SPS1 can be detected in mouse kidney and liver tissue lysates. Negligible cross-reactivity is seen against recombinant SPS2. The primary antibody was used at a 1:1000 dilution. Personal Communication, D. Hatfield, NCI, Bethesda, MD.
SEPHS1 / SPS Antibody
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Immunoprecipitation, Western Blot |
| Reactivity: | Mouse |
| Format: | Lyophilized |
$579.00each
Catalog Number: LS-C59280-100
Catalog Number: LS-C59280
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- Description
- SPS antibody LS-C59280 is an unconjugated rabbit polyclonal antibody to SPS (SEPHS1) from mouse. It is reactive with human, mouse and rat. Validated for ELISA, IP and WB.
- Application
- ELISA, IHC, IP, WB
- Reactivity
- Ms
- Predicted Reactivity
- Human, Rat
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- Selenide, water dikinase 1 | Selenophosphate synthase 1 | Selenophosphate synthetase 1 | SPS | SPS1 | SELD | Selenium donor protein 1 | SEPHS1
- Recommended Dilution: ELISA
- 1:5000 - 1:20000
- Recommended Dilution: IP
- 1:100
- Recommended Dilution: WB
- 1:500 - 1:2000
- UniProt Number
- P49903
- NCBI GenBank
- NM_012247 | NP_036379.2
- SwissProt
- P49903
Target
- Target
- Mouse SEPHS1 / SPS
- Antigen Species
- Mouse
- Epitope
- This antibody is directed against mouse SPS1. The product is delipidated and defibrinated antiserum. A BLAST analysis was used to suggest cross-reactivity with SPS1 from mouse, human and rat based on a 100% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.
- Gene Name
- SEPHS1
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
Immunogen
- Immunogen
- This antibody was prepared from whole rabbit serum produced by repeated immunizations with a full-length recombinant protein corresponding to mouse SPS1.
- Immunogen Type
- Recombinant Immunogen
Format
- Conjugate/Label
- Unconjugated
- Format
- Lyophilized
- Formulation
- Lyophilized from 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
- Concentration
- 85 mg/mL
- Volume
- 100 Microliter
- Preservative
- Yes
- Purification Method
- Unpurified (Serum / Ascites / Supernatant)
Storage & Handling
- Storage Conditions
- Store at 4°C.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl