Immunofluorescence staining of Hela cells diluted at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.The Secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
SEMA6D / Semaphorin 6D Antibody
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunofluorescence, Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$300.00each
Catalog Number: LS-C681298-50
Catalog Number: LS-C681298
For bulk orders or custom formulations:
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- Description
- Semaphorin 6D antibody LS-C681298 is an unconjugated rabbit polyclonal antibody to human Semaphorin 6D (SEMA6D). Validated for ELISA, IF and WB.
- Application
- ELISA, IF, WB
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- KIAA1479 | Semaphorin-6D | SEMA6D
- UniProt Number
- Q8NFY4
- NCBI GenBank
- NM_024966 | NP_079242.2
- SwissProt
- Q8NFY4
Target
- Target
- Human SEMA6D / Semaphorin 6D
- Antigen Species
- Human
- Gene Name
- SEMA6D
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
Immunogen
- Immunogen
- Recombinant Human Semaphorin-6D protein (913-1068AA)
- Immunogen Type
- Recombinant Protein Fragment
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.01 M PBS, pH 7.4, 0.03% ProClin™ 300, 50% Glycerol
- Preservative
- Yes
- Purification Method
- Protein G Affinity
Storage & Handling
- Storage Conditions
- Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
- Recommended Storage Buffer
- 0.01 M PBS