Immunofluorescence staining of SEMA3A in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human SEMA3A polyclonal antibody (dilution ratio 1:200) at 4°C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to Cytoplasm.
SEMA3A / Semaphorin 3A Antibody
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Paraffin |
| Reactivity: | Human |
| Format: | Liquid |
$309.00each
Catalog Number: LS-C817203-100
Catalog Number: LS-C817203
For bulk orders or custom formulations:
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- Description
- Semaphorin 3A antibody LS-C817203 is an unconjugated rabbit polyclonal antibody to human Semaphorin 3A (SEMA3A). Validated for ICC, IF and IHC.
- Application
- ICC, IF, IHC-P
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- Collapsin 1 | Coll-1 | Hsema-III | Hsema-I | Sema III | SEMAIII | SEMAL | Semaphorin III | Semaphorin-3A | Semaphorin-iii (hsema-i) | SemD | SEMA1 | SEMA3A | Semaphorin D | COLL1 | SEMAD | Semaphorin 3A | Semaphorin L | SEM3A
- Recommended Dilution: IF/ICC
- 1:100 - 1:500
- Recommended Dilution: IHC-P
- 1:50 - 1:200
- UniProt Number
- Q14563
- NCBI GenBank
- NM_006080 | NP_006071.1
- SwissProt
- Q14563
Target
- Target
- Human SEMA3A / Semaphorin 3A
- Antigen Species
- Human
- Epitope
- Human SEMA3A
- Gene Name
- SEMA3A
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Human
Immunogen
- Immunogen
- E. coli-derived Human SEMA3A fragment
- Immunogen Type
- Recombinant Protein Fragment
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, pH 7.0, 0.03% Proclin 300
- Volume
- 100 Microliter
- Preservative
- Yes
- Purification Method
- Protein A Affinity
Storage & Handling
- Storage Conditions
- Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.
- Recommended Storage Buffer
- PBS