SELPLG was immunoprecipitated using: Lane A: 0.5 mg Hela Whole Cell Lysate. 1 uL anti-SELPLG mouse monoclonal antibody and 15 ul of 50% Protein G agarose. Primary antibody: Anti-SELPLG mouse monoclonal antibody, at 1:500 dilution. Secondary antibody: Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilution. Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 43 kDa. Observed band size: 120 kDa.
SELPLG / PSGL-1 / CD162 Antibody (clone 10, Preservative Free)
Primary AntibodyLSBio Guarantee
| Antibody: | Mouse Monoclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunoprecipitation, Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$309.00each
Catalog Number: LS-C820188-100
Catalog Number: LS-C820188
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- Description
- CD162 antibody LS-C820188 is an unconjugated mouse monoclonal antibody to human CD162 (SELPLG / PSGL-1). Validated for ELISA, IP and WB.
- Application
- ELISA, IP, WB
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Alternative Names
- CD162 antigen | CLA | CD162 | PSGL1 | PSGL-1 | Selectin P ligand | SELPLG
- Recommended Dilution: ELISA
- 1:5000 - 1:10000
- Recommended Dilution: WB
- 1:500 - 1:1000
- UniProt Number
- Q14242
- NCBI GenBank
- NM_003006 | NP_002997.2
- SwissProt
- Q14242
Target
- Target
- Human SELPLG / PSGL-1 / CD162
- Antigen Species
- Human
- Epitope
- Human PSGL-1 / CD162
- Gene Name
- SELPLG
Antibody
- Host Species
- Mouse
- Clonality
- Monoclonal
- Clone
- 10
- Isotype
- IgG1
- Origin Species
- Human
Immunogen
- Immunogen
- Recombinant Human PSGL-1 / CD162 / SELPLG Protein
- Immunogen Type
- Recombinant Immunogen
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.2 µm filtered solution in PBS
- Volume
- 100 Microliter
- Preservative
- No
- Purification Method
- Protein A Affinity
Storage & Handling
- Storage Conditions
- Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.
- Recommended Storage Buffer
- PBS