Western blot using the Rabbit anti-SAE1 antibody shows detection of SAE1. Left lane (-) contains 20 µg human HeLa whole cell protein. Right lane (+) contains 20 µg human HeLa whole cell protein from cells pre-treated with phosphatase inhibitor cocktail to prevent dephosphorylation of the target. Proteins were separated on a 10% SDS-PAGE and transferred onto nitrocellulose. After blocking with 5% milk-TBST 1 hr at room temperature, the membrane was probed with the primary antibody diluted to 2 µg/mL at room temperature for 3 hr followed by washes and reaction with HRP-conjugated secondary and ECL imaging.