Western blot analysis of extracts of various cell lines, using S1PR1 antibody at 1:3000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 90s.
S1PR1 / EDG1 / S1P1 Antibody
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Western Blot |
| Reactivity: | Human, Mouse |
| Format: | Liquid |
$281.00each
Catalog Number: LS-C748008-20
Catalog Number: LS-C748008
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- Description
- S1P1 antibody LS-C748008 is an unconjugated rabbit polyclonal antibody to S1P1 (S1PR1 / EDG1) from human. It is reactive with human and mouse. Validated for WB.
- Application
- WB
- Reactivity
- Hu, Ms
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- CD363 | CD363 antigen | CHEDG1 | ECGF1 | EDG-1 | D1S3362 | EDG1 | S1P1 | S1PR1 | S1P receptor Edg-1 | S1P receptor 1
- Recommended Dilution: WB
- 1:500 - 1:2000
- UniProt Number
- P21453
- NCBI GenBank
- NM_001400 | NP_001391.2
- SwissProt
- P21453
Target
- Target
- Human S1PR1 / EDG1 / S1P1
- Antigen Species
- Human
- Gene Name
- S1PR1
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Human
Immunogen
- Immunogen
- Recombinant fusion protein containing a sequence corresponding to amino acids 303-382 of human S1PR1 (NP_001391.2). NSGTNPIIYTLTNKEMRRAFIRIMSCCKCPSGDSAGKFKRPIIAGMEFSRSKSDNSSHPQKDEGDNPETIMSSGNVNSSS
- Immunogen Type
- Fusion Protein (e.g., GST, MBP tag)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol
- Volume
- 20 Microliter
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store at -20°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- PBS