The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human S100A4 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : HeLa cell lysate Lane 2. : A549 cell lysate Lane 3. : NIH/3T3 cell lysate Lane 4. : 3T3-L1 cell lysate
S100A4 / FSP1 Antibody (clone AT1C3)
Primary AntibodyLSBio Guarantee
| Antibody: | Mouse Monoclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Flow Cytometry, Immunocytochemistry, Immunohistochemistry (general), Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$310.00each
Catalog Number: LS-C755562-50
Catalog Number: LS-C755562
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- FSP1 antibody LS-C755562 is an unconjugated mouse monoclonal antibody to human FSP1 (S100A4). Validated for ELISA, Flow, ICC and WB. Cited in 1 publication.
- Application
- ELISA, FC, ICC, IHC, WB
- Reactivity
- Hu
- Intended Use
- RUO
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Alternative Names
- 42A | 18A2 | Fibroblast-specific protein-1 | FSP1 | MTS1 | PEL98 | S100A4 | Protein Mts1 | Calvasculin | CAPL | Metastasin | p9KA | Protein S100-A4
- Recommended Dilution: IF/ICC
- 1:100 - 1:500
- Recommended Dilution: WB
- 1:1000
- UniProt Number
- P26447
- NCBI GenBank
- NM_019554, NP_062427.1
- SwissProt
- P26447
Target
- Target
- Human S100A4 / FSP1
- Antigen Species
- Hu
- Gene Name
- S100A4
Antibody
- Host Species
- Ms
- Clonality
- Monoclonal
- Clone
- AT1C3
- Isotype
- IgG2b
- Light Chain
- κ
- Origin Species
- Hu
Immunogen
- Immunogen
- Recombinant human S100A4 (1-101aa) purified from E. coli
- Immunogen Type
- Frag-RP
Format
- Conjugate/Label
- Unconj
- Format
- Liquid
- Formulation
- PBS, pH 7.4, 0.02% Sodium Azide, 10% Glycerol
- Concentration
- 1 mg/mL
- Volume
- 50 Microliter
- Preservative
- Yes
- Purification Method
- Prot A
Storage & Handling
- Storage Conditions
- Store at 4°C for up to 1 week, then aliquot and store at -20°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- PBS
Adaptive RSK-EphA2-GPRC5A signaling switch triggers chemotherapy resistance in ovarian cancer. Lidia Moyano-Galceran, Elina A Pietilä , S Pauliina Turunen , Sara Corvigno, Elisabet Hjerpe, Daria Bulanova, Ulrika Joneborg, Twana Alkasalias, Yuichiro Miki, Masakazu Yashiro, Anastasiya Chernenko, Joonas Jukonen, Madhurendra Singh, Hanna Dahlstrand, Joseph W Carlson, Kaisa Lehti. EMBO molecular medicine. 2020 Apr;12:e11177. PubMed: 32115889 PMC: PMC7136956