Western blot analysis of extracts from HepG2 cells, treated with EGF (200ng/ml, 30mins), using RSK1/2/3/4 (Phospho-Ser221/227/218/232) antibody.
RPS6KA1 / RSK1 Antibody (phospho-Ser221/227/S218/232)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunofluorescence, Western Blot |
| Reactivity: | Human, Mouse, Rat |
| Format: | Liquid |
$365.00each
Catalog Number: LS-C826663-100
Catalog Number: LS-C826663
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- Description
- RSK1 antibody LS-C826663 is an unconjugated rabbit polyclonal antibody to RSK1 (RPS6KA1) (pSer221/227/S218/232) from human. It is reactive with human, mouse and rat. Validated for ELISA, IF and WB.
- Application
- ELISA, IF, WB
- Reactivity
- Hu, Ms, Rt
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- MAPKAPK-1a | p90RSK | p90-RSK 1 | Pp90RSK1 | Ribosomal S6 kinase 1 | RSK-1 | S6K-alpha 1 | MAPKAP kinase 1a | MAPKAPK1A | p90 s6 kinase | p90S6K | RSK | RSK1 | HU-1 | p90RSK1 | RPS6KA1 | S6K-alpha-1
- Recommended Dilution: ELISA
- 1:40000
- Recommended Dilution: WB
- 1:500 - 1:1000
- UniProt Number
- Q15418
- NCBI GenBank
- NM_002953 | NP_002944.2
- SwissProt
- Q15418
Target
- Target
- Human RPS6KA1 / RSK1
- Antigen Species
- Human
- Epitope
- RSK1/2/3/4 (Phospho-Ser221/227/218/232) antibody detects endogenous levels of RSK1/2/3/4 only when phosphorylated at serine 221/227/218/232. (Human: Ser221/ Ser227/ Ser218/ Ser232; Mouse: Ser221/Ser 227/ Ser218/Ser 252)
- Gene Name
- RPS6KA1
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Human
Immunogen
- Immunogen
- The antiserum was produced against synthesized phosphopeptide derived from human RSK1/2/3/4 around the phosphorylation site of serine 221/227/218/232 (A-Y-SP-F-C).
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year.
- Recommended Storage Buffer
- PBS|150mM NaCl