Western blot of Rabbit anti-p90 RSK1 antibody. Lane 1: unstimulated HEK293T cell lysates. Lane 2: EGF stimulated HEK293T cell lysates. Load: 35 ug per lane. Primary antibody: p90 RSK1 antibody at 1:1000 for overnight at 4C. Secondary antibody: peroxidase conjugated secondary antibody and ECL. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 90 kDa, ~90 kDa for p90 RSK1. Other band(s): none.
RPS6KA1 / RSK1 Antibody (C-Terminus)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$579.00each
Catalog Number: LS-C153862-100
Catalog Number: LS-C153862
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- Description
- RSK1 antibody LS-C153862 is an unconjugated rabbit polyclonal antibody to RSK1 (RPS6KA1) (C-Terminus) from human. It is reactive with human, mouse, rat and other species. Validated for ELISA and WB.
- Application
- ELISA, WB
- Reactivity
- Hu
- Predicted Reactivity
- Bovine, Dog, Horse, Mouse, Rat
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- MAPKAPK-1a | p90RSK | p90-RSK 1 | Pp90RSK1 | Ribosomal S6 kinase 1 | RSK-1 | S6K-alpha 1 | MAPKAP kinase 1a | MAPKAPK1A | p90 s6 kinase | p90S6K | RSK | RSK1 | HU-1 | p90RSK1 | RPS6KA1 | S6K-alpha-1
- Recommended Dilution: ELISA
- 1:5000 - 1:50000
- UniProt Number
- Q15418
- NCBI GenBank
- NM_002953 | NP_002944.2
- SwissProt
- Q15418
Target
- Target
- Human RPS6KA1 / RSK1
- Antigen Species
- Human
- Epitope
- This antibody is specific for human p90 RSK1 protein. A BLAST analysis was used to suggest cross reactivity with p90 RSK1 from human, rat, mouse, horse, bovine, and dog based on 100% homology with the immunizing sequence. Cross reactivity with p90 RSK1 pS732 from other sources has not been determined.
- Gene Name
- RPS6KA1
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
Immunogen
- Immunogen
- Anti-p90 RSK1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the C-terminal end of human RSK1 protein.
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
- Concentration
- 1.2 mg/mL
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl