Anti-ROCK-2 pY256 Antibody - Western Blot. Western blot of affinity purified anti-ROCK-2 pY256 antibody shows detection of phosphorylated ROCK-2 in transfected 293T cells. Lane 1 shows lysate expressing exogenous ROCK-2 where the insert contains the transversion Y256A. Lane 2 shows the lysate expressing WT ROCK-2. A lysate containing a GST null vector also shows no specific staining (data not shown). Primary antibody was used at a 1:1000 dilution. Personal Communication, A. Papageorge and X. Qian, NCI, Bethesda, MD.

ROCK2 Antibody (phospho-Tyr256)

Primary AntibodyLSBio Guarantee

Antibody:	Rabbit Polyclonal
Applications:	Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Western Blot
Reactivity:	Human
Format:	Liquid

$579.00each

Catalog Number: LS-C60110-100

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Quantity

For bulk orders or custom formulations:

Overview

Description: ROCK2 antibody LS-C60110 is an unconjugated rabbit polyclonal antibody to human ROCK2 (pTyr256). Validated for ELISA, IHC and WB.
Application: ELISA, IHC, WB
Reactivity: Hu
Predicted Reactivity: Bovine, Chicken, Dog, Fruit Fly, Mouse, Rat, Xenopus
Intended Use: Research Use Only
Guarantees: This antibody carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names: KIAA0619 | p164 ROCK-2 | Rho kinase 2 | RhoA-associated kinase-2 | ROCK-II | Rho kinase II | Rok beta | Rok2 | ROCK2 | RockII
Recommended Dilution: ELISA: 1:2000 - 1:20000
Recommended Dilution: WB: 1:200 - 1:2000
UniProt Number: O75116
NCBI GenBank: NM_004850 | NP_004841.2
SwissProt: O75116

Target

Target: Human ROCK2
Antigen Species: Human
Epitope: This antibody is specific for human ROCK-2 protein phosphorylated at Y256. A BLAST analysis was used to suggest cross-reactivity with ROCK-2 from human, mouse, rat, chicken, dog, bovine, Xenopus and Drosophila based on a 100% homology with the immunizing sequence. Cross-reactivity with ROCK-2 from other sources has not been determined.
Gene Name: ROCK2

Antibody

Host Species: Rabbit
Clonality: Polyclonal
Isotype: IgG

Immunogen

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to residues surrounding Y256 of human ROCK-2.
Immunogen Type: Synthetic Peptide (Linear)

Format

Conjugate/Label: Unconjugated
Format: Liquid
Formulation: 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration: 1 mg/mL
Preservative: Yes
Purification Method: Affinity Purified

Storage & Handling

Storage Conditions: Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer: 0.02 M Potassium Phosphate|0.15 M NaCl