LSBio
RNF7 Antibody (aa102-113)

RNF7 Antibody (aa102-113)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Immunoprecipitation, Western Blot
Reactivity:Human
Format:Liquid
See all Human RNF7 antibodies →
$579.00each
Catalog Number: LS-C19102-100

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Overview

Description
RNF7 antibody LS-C19102 is an unconjugated rabbit polyclonal antibody to human RNF7 (aa102-113). Validated for ELISA, IHC, IP and WB. Cited in 3 publications.
Application
ELISA, IHC, IP, WB
Reactivity
Hu
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
CKII beta-binding protein 1 | CKBBP1 | RING-box protein 2 | RNF7 | Rbx2 | Regulator of cullins 2 | Ring finger protein 7 | ROC2 | Zinc RING finger protein SAG
Recommended Dilution: ELISA
1:2000 - 1:10000
Recommended Dilution: WB
1:500 - 1:1000
UniProt Number
Q9UBF6
SwissProt
Q9UBF6

Target

Target
Human RNF7
Antigen Species
Human
Epitope
Reacts with human, mouse, C.elgans and zebra fish ROC2. Cross reactivity may also occur with ROC2 from other sources. Sufficient sequence differences exist to suggest that this antibody would not react with other RING box proteins such as ROC1 and APC11.
Gene Name
RNF7

Antibody

Host Species
Rabbit
Clonality
Polyclonal

Immunogen

Immunogen
This antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 102-113 of Human ROC2 (C-terminal) coupled to KLH.
Immunogen Type
Synthetic Peptide (Linear)

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
Antiserum, 0.01% Sodium Azide
Concentration
85 mg/mL
Volume
100 Microliter
Preservative
Yes
Purification Method
Other

Storage & Handling

Storage Conditions
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid freeze-thaw cycles. Centrifuge product if not completely clear after standing at room temperature.

Rbx1, a component of the VHL tumor suppressor complex and SCF ubiquitin ligase. Kamura T, Koepp DM, Conrad MN, Skowyra D, Moreland RJ, Iliopoulos O, Lane WS, Kaelin WG, Elledge SJ, Conaway RC, Harper JW, Conaway JW. Science (New York, N.Y.). 1999;284:657-61.

PubMed: 10213691


ROC1, a homolog of APC11, represents a family of cullin partners with an associated ubiquitin ligase activity. Ohta T, Michel JJ, Schottelius AJ, Xiong Y. Molecular cell. 1999;3:535-41.

PubMed: 10230407


Cdc53/cullin and the essential Hrt1 RING-H2 subunit of SCF define a ubiquitin ligase module that activates the E2 enzyme Cdc34. Seol JH, Feldman RM, Zachariae W, Shevchenko A, Correll CC, Lyapina S, Chi Y, Galova M, Claypool J, Sandmeyer S, Nasmyth K, Deshaies RJ, Shevchenko A, Deshaies RJ. Genes & development. 1999;13:1614-26.

PubMed: 10385629 · PMC: PMC316801

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